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ODN 2088

ODN 2088 Unit size Cat. code Docs Qty Price
TLR9 antagonist - Mouse preferred
200 µg
1 mg
5 mg
tlrl-2088
+-
$173.00

TLR9 Antagonist - Inhibitory ODN, preference towards mouse

Toll-like receptor 9 (TLR9) recognizes specific unmethylated CpG oligonucleotide (ODN) sequences that distinguish microbial DNA from mammalian DNA.

Recent studies suggest the existence of DNA sequences that can neutralize the stimulatory effect of CpG ODNs [1].

These sequences are characterized by three consecutive Gs downstream of a C or A, addition of a fourth G (G-tetrads) increases the inhibitory capability.

The most potent inhibitory sequences are (TTAGGG)4 found in mammalian telomeres [2] and ODN 2088 which derives from a stimulatory ODN by replacement of 3 bases [3].

Inhibitory ODNs act by disrupting the colocalization of CpG ODNs with TLR9 in endosomal vesicles without affecting cellular binding and uptake.

 

1. Krieg AM. et al., 1998. Sequence motifs in adenoviral DNA block immune activation by stimulatory CpG motifs. Proc Natl Acad Sci USA. 95(21): 12631-6.
2. Gursel I. et al., 2003. Repetitive elements in mammalian telomeres suppress bacterial DNA-induced immune activation. J Immunol. 171(3):1393-400.
3. Stunz LL. et al., 2002. Inhibitory oligodinucleotides specifically block effects of stimulatory CpG oligonucleotides in B cells. Eur J Immunol. 32(5): 1212-22.

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Specifications

Specificity: murine TLR9 antagonist

Working concentration: 100 nM - 10 µM

Solubility:  2.5 mg/ml in water

ODN 2088 sequence
5’- tcc tgg cgg gga agt -3’ (15 mer)
Note: Bases are phosphorothioate (nuclease resistant).

Quality control:

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Contents

ODN 2088 is provided lyophilized and is available in three quantities:

  • 200 μg (41 nmol): tlrl-2088 (formerly tlrl-minhodn)
  • 1 mg (205 nmol): tlrl-2088-5 (formerly tlrl-minhodn-5)
  • 5 mg (1.025 mmol): tlrl-2088-5 (formerly tlrl-minhodn-5)
  • 1.5 ml endotoxin-free water

room temperature ODN 2088 is shipped at room temperature.

store Upon receipt, store at -20 °C.

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Citations

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