ODN 2006 (ODN 7909)

Class B CpG oligonucleotide - Human TLR9 ligand

CpG oligonucleotides (ODNs) are synthetic ODNs that contain unmethylated CpG dinucleotides in specific sequence contexts (CpG motifs).

These CpG motifs are present at a 20-fold greater frequency in bacterial DNA than in mammalian DNA. CpG ODNs activate Toll-like receptor 9 (TLR9), leading to strong immunostimulatory effects.

ODN 2006 (ODN 7909) is a class B CpG ODN and is a ligand of choice for human TLR9. Class B CpG ODNs contain a full phosphorothioate backbone with one or more CpG dinucleotides.

They strongly activate B cells but weakly stimulate IFN-α secretion in pDCs.

Specifications

Synonyms: ODN 7909, PF_3512676

Specificity: human TLR9 agonist

Working concentration: 1-5 µM

Solubility:  5 mg/ml in water

ODN 2006 sequence:
5’-tcgtcgttttgtcgttttgtcgtt-3’ (24 mer)
Note: Bases are phosphorothioate (nuclease resistant).

Quality control:
- TLR9 activity has been tested using HEK-Blue™ TLR9 cells.
- The absence of bacterial contamination (e.g. lipoproteins and endotoxins) has been confirmed using HEK-Blue™ TLR2 and HEK-Blue™ TLR4 cells.

Contents

ODN2006 is provided lyophilized and is available in three quantities:

tlrl-2006 (formerly tlrl-hodnb):

• 200 μg (25.96 nmol) lyophilized ODN2006
• 1.5 ml sterile endotoxin-free water

tlrl-2006-1 (formerly tlrl-hodnb-1):

• 1 mg (129.8 nmol) lyophilized ODN2006
• 1.5 ml sterile endotoxin-free water

tlrl-2006-5 (formerly tlrl-hodnb-5):

• 5 mg (649 nmol) lyophilized ODN2006
• 10 ml sterile endotoxin-free water

ODN 2006 (ODN 7909) is shipped at room temperature.

Upon receipt, store at -20 °C.

Description

CpG ODNs are synthetic oligonucleotides that contain unmethylated CpG dinucleotides in particular sequence contexts (CpG motifs) [1]. These CpG motifs are present at a 20-fold greater frequency in bacterial DNA compared to mammalian DNA. CpG ODNs are recognized by Toll-like receptor 9 (TLR9) leading to strong immunostimulatory effects [2].

Three types of stimulatory CpG ODNs have been identified, types A, B and C, which differ in their immune-stimulatory activities [3-4]. Type A CpG ODNs are characterized by a phosphodiester central CpG-containing palindromic motif and a phosphorothioate 3’ poly-G string. They induce high IFN-α production from plasmacytoid dendritic cells (pDC) but are weak stimulators of TLR9-dependent NF-κB signaling. Type B CpG ODNs contain a full phosphorothioate backbone with one or more CpG dinucleotides. They strongly activate B cells but stimulate weakly IFN-α secretion. Type C CpG ODNs combine features of both types A and B. They contain a complete phosphorothioate backbone and a CpG-containing palindromic motif. Type C CpG ODNs induce strong IFN-α production from pDC and B cell stimulation.

ODN 2006 is a CpG ODN type B specific for human TLR9.
 

1. Krieg, A.M. et al., 1995. CpG motifs in bacterial DNA trigger direct B-cell activation. Nature, 374(6522):546-9.
2. Bauer, S. et al., 2001. Human TLR9 confers responsiveness to bacterial DNA via species-specific CpG motif recognition. PNAS. 98(16):9237-42.
3. Krug A. et al., 2001. Identification of CpG oligonucleotide sequences with high induction of IFN- alpha/beta in plasmacytoid dendritic cells. Eur J Immunol, 31(7): 2154-63.
4. Marshall JD. et al., 2005. Superior activity of the type C class of ISS in vitro and in vivo across multiple species. DNA Cell Biol. 24(2):63-72.