ODN 2006 control (ODN 2137) - TLR9 Ligand

Negative control for ODN 2006 - Class B CpG oligonucleotide - Human TLR9-preferred ligand

ODN 2006 Control (also known as ODN 2137) is designed as a negative control for the TLR9 agonist ODN 2006, an immunostimulatory Class B CpG oligonucleotide (ODN) with a preference for human Toll-like receptor 9 (TLR9). CpG ODNs are short synthetic single-stranded DNA molecules containing unmethylated CpG dinucleotides (CpG motifs). These unmethylated CpG motifs mimic microbial DNA and act as immunostimulants via TLR9. 

Mode of action

Stimulatory CpG ODNs are internalized and activate the endosomal receptor TLR9. Activation of TLR9 triggers NF-κB- and interferon regulatory factor (IRF)-mediated pro-inflammatory responses upon the recognition of unmethylated cytosine-phosphorothioate-guanosine (CpG) forms of DNA [1-3]. Unmethylated CpG dinucleotides are a hallmark of microbial (bacterial, viral, fungal, and parasite) DNA and mitochondrial self-DNA [3, 4].

CpG Class B ODNs, such as ODN 2006, are characterized by a fully phosphorothioate-modified backbone, nuclease resistant, and one or more unmethylated CpG motifs. 
InvivoGen's ODN 2006 Control contains GpC dinucleotides instead of CpGs and does not induce TLR9 activity [5-7].

In HEK-Blue™ hTLR9 reporter cells, ODN 2006 Control does not activate hTLR9 when compared to the immunostimulatory ODN 2006.

Key features of ODN 2006 Control

• Negative control of the human TLR9-activating ODN 2006
• Synthetic ODN with unmethylated GpC motifs
• Each lot is functionally tested

Get more information about CpG ODNs Classes.

1. Kumagai Y. et al., 2008. TLR9 as a key receptor of the recognition of DNA. Adv. Drug. Deliv. Rev. 60(7):795-804.
2. Heinz L.X. et al., 2021. TASL is the SLC15A4-associated adaptor for IRF5 activation by TLR7-9. Nature. 581(7808):316-322.
3. Kayraklioglu N. et al., 2021. CpG oligonucleotides as vaccine adjuvants. DNA Vaccines: Methods and Protocols. Methods in Molecular Biology. Vol. 2197. p51-77.
4. Kumar V., 2021. The trinity of cGAS, TLR9, and ALRs: guardians of the cellular galaxy against host-derived self-DNA. Front. Immunol. 11:624597.
5. Krieg A.M. et al., 1995. CpG motifs in bacterial DNA trigger direct B-cell activation. Nature. 374(6522):546-9.
6. Vollmer J. et al., 2004. Characterization of three CpG oligodeoxynucleotide classes with distinct immunostimulatory activities. Eur. J. Immunol. 34: 251–62.
7. Rothenfusser S. et al., 2004. CpG-A and CpG-B oligonucleotides differentially enhance human peptide-specific primary and memory CD8+ T-cell responses in vitro. Blood 103:2162-9.