Engineered Human Reporter HT1080 Fibrosarcoma Cells
InvivoGen provides an HT1080-derived reporter cell line. HT1080 was originally isolated from connective tissue from a patient with fibrosarcoma. This human fibrosarcoma cell line is adherent with an epithelial morphology. HT-1080 cells have been extensively utilized for studying the mechanisms of tumor invasiveness and metastasis and for the screening of anti-cancer compounds.
InvivoGen has developed the HT1080-HMGB1-Lucia™ cell line designed for the monitoring of ferroptosis induction. Ferroptosis is a non-apoptotic, iron-dependent form of cell death, involved in many human diseases, including the pathophysiology of neurodegenerative diseases, infection, and cancer. HT1080-HMGB1-Lucia™ cells stably express an HMGB1::Lucia luciferase fusion protein combining the alarmin High-Mobility-Group-Protein B1 (HMGB1) with the secreted lucia luciferase reporter. Upon ferroptotis activation, the cells release HMGB1::Lucia which levels can be assessed by by measuring the light signal produced after the addition of QUANTI-Luc™ 4 Lucia/Gaussia , a Lucia luciferase detection reagent.
This cell line is extensively tested for viability, stability, biological activity, and absence of mycoplasma to ensure strong and reproducible results. Moreover, we provide detailed handling and experimental procedures for this cell line, to minimize the need for optimization or troubleshooting by the end-user.
Key features
- Stable expression of an HMGB1::Lucia fusion reporter gene
- Quantifiable responses upon stimulation with GPX4 inhibitors or inducers
- Functionally tested and guaranteed mycoplasma-free
Fields of Interest
- Study of Ferroptosis
- Screening of anti-cancer compounds