C-type lectin receptors (CLRs) comprise a large family of receptors that bind to carbohydrates in a calcium-dependent manner. This family includes Dectin-1, Dectin-2 and Mincle. Dectin-1 and Dectin-2 play important roles in antifungal innate immunity; they bind to beta-glucans and mannose carbohydrates, respectively. Mincle is a multi-task danger receptor that recognizes various ligands such as damaged cells, fungi, yeast, and mycobacteria. These CLRs signal through the kinase Syk and the adaptors CARD9-Bcl10-Malt1 triggering the activation of NF-κB and the subsequent production of pro-inflammatory cytokines.
InvivoGen provides a collection of HEK293-derived CLR (C-type lectin receptor) Reporter cells.
HEK-Blue™ CLR cells are designed to provide a rapid, sensitive, and reliable method to screen and validate Dectin and Mincle agonists or antagonists. They stably express human Dectin-1, human Dectin-1b, mouse Dectin-1b, mouse Dectin-2, or mouse Mincle. They also feature an NF-κB-inducible SEAP (secreted embryonic alkaline phosphatase) reporter gene. The activity of SEAP can be easily monitored in the cell supernatant using the SEAP detection reagents, QUANTI-Blue™ or HEK-Blue™ Detection.
InvivoGen's cell lines are extensively tested for viability, stability, biological activity, and absence of mycoplasma to ensure strong and reproducible results. Moreover, we provide detailed handling and experimental procedures for all cell lines, to minimize the end-user's need for optimization or troubleshooting.
InvivoGen also offers a murine macrophage-derived CLR reporter cell line that endogenously expresses Dection and Mincle and a collection of CLR ligands.
