HEK-Blue™ mDectin-2 Cells

HEK-Blue™ mDectin-2 Cells Unit size Cat. code Docs Qty Price
Murine Dectin-2 / NF-κB / SEAP reporter HEK293 cells
3-7 x 10e6 cells

SEAP Reporter 293 cells expressing the mouse Dectin-2 gene

HEK-Blue™ mDectin-2 cells stably express the mouse Dectin-2 gene, as well as the genes of the Dectin-2 signaling pathway. They also stably express an NF-kB-inducible secreted embryonic alkaline phosphatase (SEAP) reporter gene.

These reporter cell lines are activated by Dectin-2 ligands. They do not respond to other CLR ligands, such as trehalose-6,6-dibehenate (TDB), a Mincle ligand.

Levels of SEAP can be easily determined with HEK-Blue™ Detection, a cell culture medium that allows for real-time detection of SEAP. SEAP activity can also be assessed using the alkaline phosphatase detection reagent, QUANTI-Blue™.

HEK-Blue™ mDectin-2 and HEK-Blue™ Null1-v cells (parental cell line) were stimulated with various CLR agonists: zymosan depleted (100 µg/ml), zymosan (10 µg/ml), furfurman (10 µg/ml), HKCA (1x108 cells), HKSC (1x108 cells), TDB (10 µg/ml), and TNF-α (100 ng/ml). TNF-α, an NF-κB activator, was used as a positive control. NF-κB-induced SEAP activity was assessed using HEK-Blue™ Detection and by reading the optical density (OD) at 655 nm. Non-induced cells (NI) served as negative controls.

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Required Selective Antibiotics: Blasticidin, Puromycin and HEK-Blue™ CLR Selection.

Growth medium: DMEM (4.5 g/l glucose), 10% (v/v) fetal bovine serum (FBS), 50 U/ml penicillin, 50 µg/ml streptomycin, 100 µg/ml Normocin™, 2 mM L-glutamine.

Guaranteed mycoplasma-free.

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Shipped on dry ice

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Quality Control
HEK-Blue™ mDectin-2 cells have been stimulated by various pathogen recognition receptor (PRR) agonists. As expected, Dectin-2 agonists induced the production of SEAP. The cell surface expression of murine Dectin-2 (mDectin-2) in this cell line has been validated using fluorescence-activated cell sorting (FACS). The stability of this cell line for 20 passages following thawing has been verified.

Specificity of HEK-Blue™ mDectin-2 cells
As their parental cell line, HEK293 cells, express endogenous levels of TLR3, TLR5 and NOD1, HEK-Blue™ mDectin-2 cells will respond to TLR3, TLR5 and NOD1 agonists, such as poly(I:C), flagellin and C12‑iE‑DAP, respectively. In order to identify Dectin-2-specific responses, we recommend to use HEK-Blue™ Null1-v cells as a control cell line.

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