Mouse Dectin-2 Reporter HEK 293 Cells

SEAP Reporter 293 cells expressing the murine Dectin-2 gene

HEK-Blue™ mDectin-2 cells have been engineered to express mouse Dectin-2 and a secreted alkaline phosphatase (SEAP) in response to stimulation by Dectin-2 ligands.

Dectin-2 is a C-type lectin receptor (CLR) that binds high mannose-type carbohydrates and has been demonstrated to be the functional receptor for α-mannans.  This receptor plays an important role in antifungal innate immunity [1, 2].

HEK-Blue™ mDectin-2 cells are derived from the human embryonic kidney HEK293 cells which do not naturally express Dectin-2. We have engineered HEK-Blue™ cells that stably express high levels of murine Dectin-2 as well as the genes of the Dectin-2 signaling pathway. These cells also stably express an NF-κB-inducible SEAP reporter gene. Levels of SEAP are readily measurable in real-time by a simple colorimetric assay with HEK-Blue™ Detection or QUANTI-Blue™.

HEK-Blue™ mDectin-2 cells reporter cell lines respond to Dectin-2 ligands, such as Furfurman.

They do not respond to other CLR ligands, such as trehalose-6,6-dibehenate (TDB), a Mincle ligand.

To identify Dectin-2-specific responses, we recommend to use HEK-Blue™ Null1-v cells as a control cell line.

References:

1.  Hou H. et al, 2017. C-type Lectin Receptor: Old Friend and New Player. Med Chem. 2017;13(6):536-543. 
2. Wang HL. et al., 2017. Ligation of Dectin-2 with a novel microbial ligand promotes adjuvant activity for vaccination. PLoS Pathog. 213(8):e1006568.