Mouse Mincle Reporter HEK 293 Cells

SEAP Reporter 293 cells expressing the murine Mincle gene

HEK-Blue™ mMincle cells have been engineered to express a secreted alkaline phosphatase (SEAP) in response to stimulation by Mincle ligands.

Mincle is a C-type lectin receptor (CLR) that recognizes a variety of exogenous and endogenous stimuli, such as mycobacteria, certain fungi and necrotic cells[1, 2].

HEK-Blue™ mMincle cells are derived from the human embryonic kidney HEK293 cells which do not naturally express Mincle. We have engineered HEK-Blue™ cells that stably express high levels of murine Mincle as well as the genes of the Mincle-NF-κB signaling pathway. These cells also stably express an NF-κB-inducible SEAP reporter gene. Levels of SEAP are readily measurable in real-time by a simple colorimetric assay with HEK-Blue™ Detection or QUANTI-Blue™

HEK-Blue™ mMincle cells are activated by Mincle ligands, such as trehalose-6,6-dibehenate (TDB) and heat-killed M. tuberculosis (HKMT).

They do not respond to other CLR ligands such as zymosan, a Dectin-1 and TLR2 ligand.

To identify Mincle-specific responses, we recommend to use HEK-Blue™ Null1-v cells as a control cell line.

Key features

• Readily assessable NF-κB-SEAP reporter activity
• Convenient readout using QUANTI-Blue™ Solution
• High sensitivity to Mincle ligands
• Stability guaranteed for 20 passages

HEK-Blue™ hMincle cells expressing the human Mincle gene (hMincle) are available upon request. Contact us for more information.

References:

1. Yamasaki S. et al., 2009​. C-type lectin Mincle is an activating receptor for pathogenic fungus, Malassezia. PNAS 106(6): 1897–1902. 
2. Brown GD. 2008.  Sensing necrosis with Mincle. Nature Immunol. 9:1099-1100.