HKMT - Mincle & TLR2 Agonist

Heat-killed Mycobacterium tuberculosis

ABOUT

Heat-killed Mycobacterium tuberculosis

HKMT is a heat-killed preparation of the avirulent strain of Mycobacterium tuberculosis H37 Ra.

HKMT is sensed by Mincle which recognizes the mycobacterial cell wall glycolipid TDM. HKMT also possesses a large repertoire of TLR2 ligands, such as lipoproteins and lipomannan. Upon HKMT sensing, both Mincle and TLR2 lead to the activation of NF-κB.

 

References:

1. Ishikawa E. et al., 2009. Direct recognition of the mycobacterial glycolipid, trehalose dimycolate, by C-type lectin Mincle. 206(13):2879-88.
2. Bhatt K & Salgame P., 2007. Host innate immune response to Mycobacterium tuberculosis. J Clin Immunol 27(4): 347–362.

All products are for research use only, and not for human or veterinary use.

SPECIFICATIONS

Specifications

Source
Mycobacterium tuberculosis H37 Ra
Product concentration

10 - 100 µg/ml when used as a Mincle ligand
100 ng - 10 µg/ml when used as a TLR2 ligand

Working concentration

10 - 100 µg/ml as Mincle ligand, 100 ng - 10 µg/ml as TLR2 ligand

Quality control

Each lot is functionally tested and validated.

CONTENTS

Contents

  • Product: 
    HKMT
  • Cat code: 
    tlrl-hkmt-1
  • Quantity: 
    10 mg
Includes:

1.5 ml endotoxin-free water

Notes:

Heat-killed preparation of Mycobacterium tuberculosis H37Ra (HKMT) is provided lyophilized

Shipping & Storage

  • Shipping method:  Room temperature
  • Storage:

    • 4°C

    Caution:

    • Avoid repeated freeze-thaw cycles

Details

Responses to M. tuberculosis-derived ligands
Responses to M. tuberculosis-derived ligands.


RAW-Blue™ cells, and HEK-Blue™ hMincle, HEK-Blue™ TLR2 or HEK-Blue™ TLR4 cells, which express the human mincle, TLR2 or TLR4 gene, respectively, were stimulated with 10 µg/ml of M. tuberculosis-derived ligands. After 24h incubation, NF-κB activation was assessed by measuring the levels of SEAP using the QUANTI-Blue™ assay.

DOCUMENTS

Documents

HKMT

Technical Data Sheet

Safety Data Sheet

Certificate of analysis

Need a CoA ?

CUSTOMER SERVICE & TECHNICAL SUPPORT

Question about this product ?