HKMT
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Cat.code:
tlrl-hkmt-1
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ABOUT
Heat-killed Mycobacterium tuberculosis
HKMT is a heat-killed preparation of the avirulent strain of Mycobacterium tuberculosis H37 Ra.
HKMT is sensed by Mincle which recognizes the mycobacterial cell wall glycolipid TDM. HKMT also possesses a large repertoire of TLR2 ligands, such as lipoproteins and lipomannan. Upon HKMT sensing, both Mincle and TLR2 lead to the activation of NF-κB.
References:
1. Ishikawa E. et al., 2009. Direct recognition of the mycobacterial glycolipid, trehalose dimycolate, by C-type lectin Mincle. 206(13):2879-88.
2. Bhatt K & Salgame P., 2007. Host innate immune response to Mycobacterium tuberculosis. J Clin Immunol 27(4): 347–362.
All products are for research use only, and not for human or veterinary use.
SPECIFICATIONS
Specifications
10 - 100 µg/ml when used as a Mincle ligand
100 ng - 10 µg/ml when used as a TLR2 ligand
10 - 100 µg/ml as Mincle ligand, 100 ng - 10 µg/ml as TLR2 ligand
Each lot is functionally tested and validated.
CONTENTS
Contents
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Product:HKMT
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Cat code:tlrl-hkmt-1
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Quantity:10 mg
1.5 ml endotoxin-free water
Heat-killed preparation of Mycobacterium tuberculosis H37Ra (HKMT) is provided lyophilized
Shipping & Storage
- Shipping method: Room temperature
- 4°C
- Avoid repeated freeze-thaw cycles
Storage:
Caution:
Details
Responses to M. tuberculosis-derived ligands.
RAW-Blue™ cells, and HEK-Blue™ hMincle, HEK-Blue™ TLR2 or HEK-Blue™ TLR4 cells, which express the human mincle, TLR2 or TLR4 gene, respectively, were stimulated with 10 µg/ml of M. tuberculosis-derived ligands. After 24h incubation, NF-κB activation was assessed by measuring the levels of SEAP using the QUANTI-Blue™ assay.
DOCUMENTS
Documents
Technical Data Sheet
Safety Data Sheet
Certificate of analysis
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