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pFUSEN-Lucia-hG1e2Fc

pFUSEN-Lucia-hG1e2Fc Unit size Cat. code Docs Qty Price
Engineered Fc Regions Lucia-Tagged
20 µg
pfcn-lchg1e2
+-
$555.00

Production of Lucia luciferase-tagged IgG1e2-Fc fusion protein

pFUSEN-Lucia-hG2Fc is a cloning plasmid for the generation of a Lucia luciferase-tagged engineered Fc-fusion protein expressing the Fc region (CH2 and CH3 domains) of the mutated human IgG1 heavy chain and the hinge region fused to the N-terminus of a protein of interest.

Lucia is a secreted luciferase reporter protein with advantageous characteristics when associated with Fc-fusion proteins.

It possesses superior carrier ability for excellent secretion of the chimeric protein. It provides a simple means to screen for recombinant clones and it minimally affects the activity of the protein of interest.

Human IgG1e2-Fc contains mutations in the site of interaction of IgG1 with FcRn that enhance the plasma half-life by increasing the affinity of IgG1 for FcRn.

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Specifications

Isotype: IgG1 human

Mutations: M252Y/S254T/T256E + H433K/N434F

Characteristics:

  • Increased binding to neonatal FcR (FcRn)
  • Increased half-life

Selectable with Zeocin™ in E. coli and mammalian cells.
For transient or stable transfection.

These products are covered by a Limited Use License (See Terms and Conditions).

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Contents

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Details

pFUSEN-Lucia-Fc Map

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