Invivogen
Menu

pFUSEN-Lucia-hG1e2Fc

pFUSEN-Lucia-hG1e2Fc Unit size Cat. code Docs Qty Price
Engineered Fc Regions Lucia-Tagged
20 µg
pfcn-lchg1e2
+-
$536.00

Production of Lucia luciferase-tagged IgG1e2-Fc fusion protein

pFUSEN-Lucia-hG2Fc is a cloning plasmid for the generation of a Lucia luciferase-tagged engineered Fc-fusion protein expressing the Fc region (CH2 and CH3 domains) of the mutated human IgG1 heavy chain and the hinge region fused to the N-terminus of a protein of interest.
Lucia is a secreted luciferase reporter protein with advantageous characteristics when associated with Fc-fusion proteins. It possesses superior carrier ability for excellent secretion of the chimeric protein. It provides a simple means to screen for recombinant clones and it minimally affects the activity of the protein of interest.

Human IgG1e2-Fc contains mutations in the site of interaction of IgG1 with FcRn that enhance the plasma half-life by increasing the affinity of IgG1 for FcRn.

Back to the top

Specifications

Isotype: IgG1 human
Mutations: M252Y/S254T/T256E + H433K/N434F
Characteristics:
- Increased binding to neonatal FcR (FcRn)
- Increased half-life

Selectable with Zeocin™ in E. coli and mammalian cells.
For transient or stable transfection.

These products are covered by a Limited Use License (See Terms and Conditions).

Back to the top

Contents

• 20 μg of lyophilized DNA
• 4 pouches of E. coli Fast-Media® Zeo (2 TB and 2 Agar)

Back to the top

Details

pFUSEN-Lucia-Fc Map

Back to the top

Customer Service
& Technical Support

CAPTCHA
In order to prevent automated spam submissions, please answer the following question:
1 + 4 =
Solve this simple math problem and enter the result. E.g. for 1+3, enter 4.
Shopping cart is empty