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Fc-fusion protein expression plasmid - Engineered human IgG1
pFUSEN-hG1e2Fc is a cloning plasmid for the generation of a Fc-fusion protein expressing the Fc region (CH2 and CH3 domains) of the mutated human IgG1 heavy chain and the hinge region fused to the N-terminus of a protein of interest..
The engineered hIgG1e2 Fc contains the following mutations: M252Y/S254T/T256E and H433K/N434F.
Human IgG1e2-Fc contains mutations in the site of interaction of IgG1 with FcRn that enhance the plasma half-life by increasing the affinity of IgG1 for FcRn.
Isotype: IgG1 human
Mutations: M252Y/S254T/T256E + H433K/N434F
- Increased binding to neonatal FcR (FcRn)
- Increased half-life
Selectable with Zeocin™ in E. coli and mammalian cells.
For transient or stable transfection.
• 20 μg of lyophilized DNA
• 4 pouches of E. coli Fast-Media® Zeo (2 TB and 2 Agar)
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