HepG2 hepatoma cell lines

Engineered Human Reporter HepG2 Liver Carcinoma Cells

HepG2 human hepatoma cells were originally isolated from the liver tissue of a patient with a well-differentiated hepatocellular carcinoma. This adherent-growing cell line has become a commonly used cell model to study cellular responses to infection with hepatotropic pathogens [1]. HepG2 cells are also used in biomedical research to investigate drug metabolism in the liver. Since they endogenously express a variety of antioxidant and xenobiotic metabolizing enzymes, HepG2 cells are a suitable tool for detecting environmental pollutants and dietary-derived cytotoxic and genotoxic compounds [2]. 


Therefore, HepG2 cells are a great tool to

study hepatocarcinogenesis and liver diseases

- examine hepatotropic pathogen infections

investigate drug metabolism and cell signaling pathways

screen for gut microbiota-derived or xenobiotic ligands


All our cell lines are extensively tested for viability, stability, biological activity, and absence of mycoplasma to ensure strong and reproducible results. Moreover, we provide detailed handling and experimental procedures for all cell lines, to minimize the need for optimization or troubleshooting by the end-user.

The collection comprises

  • NF-κB / IRF Reporter Cells
  • AhR Reporter Cells

Key Features

  • Stable expression of one or two reporter systems 
  • Quantifiable responses upon stimulation with xenobiotics, synthetic dietary-derived, or endogenous AhR agonists
  • Functionally tested and guaranteed mycoplasma-free


InvivoGen's HepG2-Dual™ reporter cells feature two inducible reporter genes for SEAP (secreted embryonic alkaline phosphatase) and Lucia luciferase. As a result, these cells allow the simultaneous study of the NF-κB and/or the IRF pathways, by assessing the activity of SEAP or the secreted Lucia luciferase. Both reporter proteins are readily measurable in the cell culture supernatant when using QUANTI-Blue™, a SEAP detection reagent, and QUANTI-Luc™ 4 Lucia/Gaussia, a Lucia luciferase detection reagent. 

The HepG2-Lucia™ AhR cells express the aryl hydrocarbon receptor (AhR) as well as the inducible reporter gene for Lucia luciferase. As a result, these cells allow the study of the AhR genomic signaling pathway, by assessing the activity of secreted Lucia luciferase. The reporter protein is readily measurable in the cell culture supernatant when using QUANTI-Luc™ 4 Lucia/Gaussia, a Lucia luciferase detection reagent. 



1. Gural N. et al. 2017. Engineered livers for infectious diseases. Cell Mol Gastroenterol Hepatol. 5:131-44.
2. Mersch-Sundermann V, et al., 2004. Use of a human-derived liver cell line for the detection of cytoprotective, antigenotoxic and cogenotoxic agents. Toxicology. 198(1-3):329-40.

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