Engineered Mouse Reporter J774 Macrophages
InvivoGen provides a J774.1-derived dual reporter cell line. J774.1 was originally isolated from the ascites of an adult, female BALB/cN mouse with reticulum cell sarcoma. This mouse cell line is adherent and expresses characteristics of macrophages. J774.1 cells are active in antibody-dependent phagocytosis and continuously synthesize interleukin-1 beta (IL-1β). J774.1 is a well-established model system in cell biology and immunology. J774.1 cells express a variety of pattern recognition receptors (PRRs), including Toll-like receptors (TLRs), C-type lectin receptors (CLRs), and RIG-I-like receptors (RLRs).
InvivoGen has developed J774-Dual cells designed for the study of the NF-κB and/or IRF pathways. This cell line features two inducible reporter constructs allowing the simultaneous monitoring of NF-κB and IRF activation by assessing the activity of secreted embryonic alkaline phosphatase (SEAP) and the secreted Lucia luciferase, respectively. Both reporter proteins are readily measurable in the cell culture supernatant when using QUANTI-Blue™, a SEAP detection reagent, and QUANTI-Luc™ 4 Lucia/Gaussia, a Lucia luciferase detection reagent.
This cell line is extensively tested for viability, stability, biological activity, and absence of mycoplasma to ensure strong and reproducible results. Moreover, we provide detailed handling and experimental procedures for this cell line to minimize the need for optimization or troubleshooting by the end-user.
Key Features
- Stable expression of two reporter systems
- Quantifiable responses upon stimulation with pathogen/damage-associated molecular patterns (PAMPs/DAMPs) or cytokines
- Functionally tested and guaranteed mycoplasma-free
Fields of Interest
- Study of phagocytosis
- Study of PRR responses
