Human & mouse genes in pUNO or pDUO
InvivoGen provides an expanding collection of over 1,800 full-length sequenced genes, mainly involved in immunity and cancer.
Fully sequenced genes
- Genes from different species are available (human, mouse, bovine, pork, rat, etc.)
- Provided as open reading frames (ORFs) from the ATG to the Stop codon, excluding introns and untranslated regions
- All sequences are available online on the gene page or can be emailed upon request
As the ORFs are amplified from cDNA libraries, a variant form (allele) is sometimes obtained. Most of the variations have been reported in Genbank.& Genes for naturally secreted proteins include the native signal sequence. Engineered proteins meant to be secreted (such as particular fragments of longer proteins, like angiostatic proteins) may include the signal sequence of the human IL-2.
pUNO & pDUO plasmids
- Expression of one (pUNO) or two (pDUO) genes of interest
- Selectable in both bacterial and mammalian cells
- Provided with a mammalian expression cassette
- Simple to subclone due to unique restriction sites
- Control plasmids pUNO-mcs and pDUO-mcs featuring multiple cloning sites (mcs) are also available
Most of these plasmids are selectable with Blasticidin (pUNO1/pDUO). Some are also provided with a Zeocin® (pUNO2), Hygromycin (pUNO3/pDUO2), or Puromycin (pUNO4) resistance.
Choice of engineered genes
- Fusion genes (ie. IL12, IL39, ENDO::ANGIO) with the 'elasti' linker (VPGVG)
- Genes with selected modifications (gain/loss of function mutations, disease-related variations, etc.)
- HA-tagged genes for specific detection by Western blot
- CpG-free genes
Our library contains genes from a plethora of research fields including COVID-19, autophagy, innate & adaptive immunity, and cancer. An extensive list of COVID-19-related genes. Each product is provided as lyophilized DNA (20 µg) together with 2 x 1 ml blasticidin, 1 ml Zeocin®, 1 ml Hygromycin B Gold, or 1 ml Puromycin.
