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pUNO-mcs

pUNO1-mcs Unit size Cat. code Docs Qty Price
20 µg
puno1-mcs
+-
$257.00
pUNO1-mcs purchased with a pUNO1-[gene] Unit size Cat. code Docs Qty Price
20 µg
puno1-mcs.
+-
$200.00
pUNO1ss-mcs Unit size Cat. code Docs Qty Price
pUNO1-mcs with hIL2 signal sequence, for cloning gene fragments lacking the secretion signal
20 µg
puno1ss-mcs
+-
$257.00
pUNO2-mcs Unit size Cat. code Docs Qty Price
20 µg
puno2-mcs
+-
$257.00
pUNO3-mcs Unit size Cat. code Docs Qty Price
20 µg
puno3-mcs
+-
$257.00

pUNO-mcs plamids were designed to serve as control vectors for the pUNO plasmid family that feature a wide choice of native and fusion genes.

They carry a multiple cloning site (MCS) that features commonly used restriction sites to allow convenient insertion of an open reading frame.

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Specifications

pUNO1: selectable with Blasticidin in mammalian cells and E.coli

pUNO2: selectable with Zeocin™ in mammalian cells and E.coli

pUNO3: selectable with Hygromycin in mammalian cells and E.coli

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Contents

pUNO1-mcs:

  • 20 µg of lyophilized DNA
  • 2 x 1 ml of blasticidin at 10 mg/ml

 

pUNO2-mcs:

  • 20 µg of lyophilized DNA
  • 4 pouches of E. coli Fast-Media®  Zeo (2 TB and 2 Agar)
  • 1 ml of Zeocin™ at 100 mg/ml

 

pUNO3-mcs:

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Details

pUNO1 backbone

The gene of interest is under the control of of the strong and ubiquitous mammalian promoter, EF1α/HTLV. pUNO plasmids can be used for in vitro or in vivo transfection experiments.

pUNO-mcs plamids carry a multiple cloning site (MCS) that features commonly used restriction sites to allow convenient insertion of an open reading frame.

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Citations

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