InvivoGen provides a collection of recombinant isotype control monoclonal antibodies (mAbs) designed to help differentiate non-specific background from specific antibody signal. In general, isotype controls should match the primary antibody's host, isotype and subclass, method of production, and working concentration.
The mouse isotype control mAb family comprises the variable region of an E.coli anti-β-galactosidase (β-Gal) mAb and the murine constant region of different native (IgG1, IgG2a, IgG2b) or engineered (IgG1e3) isotypes. These mAbs differ in their effector functions, such as complement-dependent cytotoxicity (CDC) and antibody-dependent cell-mediated cytotoxicity (ADCC).
Our mouse isotype controls are either produced in hybridoma cells or Chinese hamster ovary (CHO) cells using recombinant technology. They can be used as controls for blocking, neutralization, depletion, or activation assays in vivo or in vitro, as well as for other applications, including immunofluorescence (IF), immunocytochemistry (ICC), immunoprecipitation (IP), flow cytometry and ELISA.