Murine Isotype Controls

InvivoGen provides a collection of isotype control monoclonal antibodies (mAbs) designed to help differentiate non-specific background from specific antibody signal. In general, isotype controls should match the primary antibody's host, isotype and subclass, method of production, and working concentration.

The murine isotype control mAb family comprises the variable region of an E.coli anti-β-galactosidase (β-Gal) mAb and the constant region of different native (IgG1, IgG2a, IgG2b) or engineered (IgG1e3) isotypes. These mAbs differ in their effector functions, such as complement-dependent cytotoxicity (CDC) and antibody-dependent cell-mediated cytotoxicity (ADCC).

 Read our review on Antibody Isotypes

Our murine isotype controls are either produced in hybridoma cells, or in Chinese hamster ovary (CHO) cells using recombinant technology. They can be used for blocking, neutralization, depletion or activation assays in vivo or in vitro, as well as for other applications, including immunofluorescence (IF), immunocytochemistry (ICC), immunoprecipitation (IP), flow cytometry and ELISA.

Note: InvivoGen also provides Anti-β-Gal-mIgG1 and Mouse Control-IgG2b upon request.