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Recombinant mouse IgG2a isotype control

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Anti-β-Gal-mIgG2a InvivoFit™

β-galactosidase antibody; Mouse IgG2a isotype control

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1 mg

10 mg

50 mg

bgal-mab10-1
+-
$335

Recombinant mouse IgG2a isotype control mAb against E. coli β-galactosidase

InvivoGen’s Anti-β-Gal-mIgG2a InvivoFit™ antibody
InvivoGen’s Anti-β-Gal-mIgG2a InvivoFit™ antibody

InvivoGen provides a recombinant anti-β-Gal-mIgG2a monoclonal antibody (mAb). It is expressed and produced in chinese hamster ovary (CHO) cells, ensuring reliability and lot-to-lot reproducibility. Thereby, common hybridoma-related drawbacks such as the generation of non-relevant mAbs containing aberrant light chains are avoided. This control antibody targets E. coli's β-galactosidase (β-Gal), an antigen that is not present in mice. In addition, it displays very high ADCP (antibody-dependent cell-mediated phagocytosis), high ADCC (antibody-dependent cell-mediated cytotoxicity) and high CDC (complement-dependent cytotoxicity) effector functions [1].

 

The sequence of Anti-β-Gal-mIgG2a InvivoFit™ is 100% murine (constant and variable regions), as the original clone (clone T9C6) was raised in mice using a proprietary method. This feature allows for reduced immunogenicity and risks of fatal hypersensitivity reactions upon repeated mAb injections into mice [2]. 

 

Anti-β-Gal-mIgG2a InvivoFit™ is provided in the InvivoFit™ grade, suitable for in vivo studies. All InvivoFit™ products are handled in a clean room, filter-sterilized, and tested for bacterial contaminants. Additionally, this grade guarantees low levels of endotoxins (<1 EU/ml). The buffer formulation is specifically adapted for in vivo studies.

 

Key features:

  • Targets E. coli β-galactosidase antigen 
  • Sequence is 100% murine
  • Murine IgG2 isotype (constant region)
  • Very high ADCP, high ADCC, and high CDC
  • Free from non-relevant mAbs found in hybridoma-based productions
  • Produced in animal-free facilities and defined media
  • Low aggregation < 5%
  • InvivoFit™ grade

 

References:

1. Nimmerjahn F. et al., 2005. FcgammaRIV: a novel FcR with distinct IgG subclass specificity. Immunity. 2005 Jul;23(1):41-51.

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Specifications

Target: E. coli β-galactosidase (β-Gal)

Specificity: no cross-reaction with murine proteins

Clone: T9C6

Source: CHO cells

Isotype: Murine IgG2a, kappa

Formulation of Anti-β-Gal-mIgG2a InvivoFit™: Lyophilized from 0.2 µm filtered solution in a sodium phosphate buffer with 5% saccharose

Application: Negative control for murine IgG2a antibodies

Quality control:

  • The absence of binding was confirmed by flow cytometry.
  • The complete sequence of this antibody has been verified.
  • The absence of bacterial contamination (e.g. lipoproteins and endotoxins) has been confirmed using HEK-Blue™ TLR2 and HEK‑Blue™ TLR4 cells.
  • The endotoxin level is <1 EU/mg (determined by a LAL assay).
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Contents

Anti-β-Gal-mIgG2a InvivoFit™ is filter-sterilized (0.2 µm), endotoxin-free, azide-free, and lyophilized.  

 

This product is available in three pack sizes:

  • bgal-mab10-1​: 1 mg
  • bgal-mab10-10​: 10 mg
  • bgal-mab10-50​: 50 mg (5 x 10 mg)

 

room temperature The product is shipped at room temperature.

store Store lyophilized antibody at -20 °C.

stability Lyophilized product is stable for at least 1 year.

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InvivoFit™

InvivoFit™ is a high-quality standard specifically adapted for in vivo studies. InvivoFit™ products are filter-sterilized (0.2 µm) and filled under strict aseptic conditions in a clean room. The level of bacterial contaminants (endotoxins and lipoproteins) in each lot is verified using a LAL assay and a TLR2 and TLR4 reporter assay.

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Citations

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