Recombinant Mouse IgG2a Isotype Control Antibody

Recombinant mouse IgG2a isotype control mAb against E. coli β-galactosidase

InvivoGen provides a recombinant anti-β-Gal-mIgG2a monoclonal antibody (mAb). It is expressed and produced in chinese hamster ovary (CHO) cells, ensuring reliability and lot-to-lot reproducibility. Thereby, common hybridoma-related drawbacks such as the generation of non-relevant mAbs containing aberrant light chains are avoided. This control antibody targets E. coli's β-galactosidase (β-Gal), an antigen that is not present in mice. In addition, it displays very high ADCP (antibody-dependent cell-mediated phagocytosis), high ADCC (antibody-dependent cell-mediated cytotoxicity) and high CDC (complement-dependent cytotoxicity) effector functions [1].

The sequence of Anti-β-Gal-mIgG2a InvivoFit™ is 100% murine (constant and variable regions), as the original clone (clone T9C6) was raised in mice using a proprietary method. This feature allows for reduced immunogenicity and risks of fatal hypersensitivity reactions upon repeated mAb injections into mice [2].

Key features:

• Targets E. coli β-galactosidase antigen
• Sequence is 100% murine
• Murine IgG2 isotype (constant region)
• Very high ADCP, high ADCC, and high CDC
• Free from non-relevant mAbs found in hybridoma-based productions
• Produced in animal-free facilities and defined media
• Low aggregation < 5%
• InvivoFit™ grade

This antibody is also available in bulk quantity, please contact us

References:

1. Nimmerjahn F. et al., 2005. FcgammaRIV: a novel FcR with distinct IgG subclass specificity. Immunity. 2005 Jul;23(1):41-51.