InvivoGen introduces secreted ACP5, a novel reporter protein
ACP5, also known as tartrate-resistant acid phosphatase (TRAP or TRAPase) is a metalloprotein enzyme, usually located in the lysosome. InvivoGen has developed a secreted version of ACP5 that can be used as a reporter gene.
The level of ACP5 activity in the cell culture supernatant is easily quantified using QUANTI-Star™, our easy-to-use detection reagent. QUANTI-Star™ contains an ACP5- specific colorimetric substrate, which harbors a phosphate group. Cleavage of this phosphate group by secreted (s)ACP5 leads to the formation of a soluble yellow reaction product, which can be detected at 405 nm using a microplate reader or spectrophotometer.
It is combinable with other reporter genes, such as SEAP or Lucia luciferase.
ACP5 secretion can be assessed without disturbing cells, leaving them intact for further experimentation. It does not require the preparation of cell lysates and can be used for kinetic studies.
To support your research, InvivoGen provides a growing collection of ACP5 products:
- Secreted ACP5 reporter detection reagent for real-time detection and quantification of ACP5 - QUANTI-Star™
- Plasmid for in vivo & in vitro use
- ACP5 reporter cell line - HEK-Lucia-Star™ ARE cell line (Nrf2 KEAP1 pathway)
Key features of ACP5 Reporter Systems
- Easy-to-use reagent available
- Combinable with other reporter systems
- Adaptable for high-throughput screens (HTS)
- Quantifiable and reliable results
- ACP5 activity is measurable in the supernatant without disturbing cells
Our HEK-Lucia-Star™ ARE cell line was designed to measure oxidative stress and its effect on cell viability. These cells constitutively express the secreted version of ACP5. The constant level of ACP5 activity in the cell culture supernatant is easily quantified by reading the OD. The signal corresponds to the amount of APC5 protein expressed, indicating healthy cell culture conditions. Upon cell death, the ACP5 signal is lost. The decrease of released ACP5 in the supernatant correlates to induced cell death and thus, provides information about the viability of your cell culture.