HEK293 cell line collection to study dsRNA signaling pathways of TLR3, RIG-I, and MDA5
Upon recognition of viral or synthetic double-stranded (ds)RNA, the major pattern recognition receptors (PRRs) Toll-like receptor 3 (TLR3), Retinoic acid-inducible protein 1 (RIG-I), and Melanoma differentiation-associated gene 5 (MDA5) collectively establish an antiviral host response. They mediate the transcriptional induction of type I interferons (IFNs), proinflammatory cytokines, or even cell death.
To facilitate the monitoring of dsRNA-mediated responses, InvivoGen has developed HEK-Dual™-derived cell lines to exclusively assess the role of one human dsRNA sensor.
They express two reporter proteins, an NF-κB-inducible secreted embryonic alkaline phosphatase (SEAP) reporter and an interferon regulatory factor (IRF)-inducible Lucia® luciferase reporter.
HEK-Dual™ RNA-Null cells have no endogenous expression of three important dsRNA sensors Toll-like receptor 3 (TLR3), Retinoic acid-inducible protein 1 (RIG-I), and Melanoma differentiation-associated gene 5 (MDA5).
In HEK-Dual™ RNA-hTLR3 cells, HEK-Dual™ RNA-hRIG-I cells, or HEK-Dual™ RNA-hMDA5 cells, the corresponding PRR was reintroduced, allowing the study of senor-specific responses without interference from the other two PRRs.
Key features
- Strong NF-κB and IRF responses depending on the ligand
- Simultaneously assessable NF-κB-SEAP and IRF-Lucia® reporter activity
- Convenient readout using QUANTI-Blue™ and QUANTI-Luc™ 4 Lucia/Gaussia
- Stability guaranteed for 20 passages
These cell lines are extensively tested for viability, stability, biological activity, and absence of mycoplasma to ensure strong and reproducible results. Moreover, we provide detailed handling and experimental procedures for all cell lines, to minimize the need for optimization or troubleshooting by the end-user.
The activity of the reporter proteins, SEAP and Lucia luciferase, can be conveniently assayed in the cell supernatants using the SEAP detection reagents QUANTI-Blue™ Solution and the Lucia luciferase detection reagent, QUANTI-Luc™ 4 Lucia/Gaussia. InvivoGen also offers a comprehensive collection of STING products, including TLR3 ligands, RLR ligands, TLR3 and RLR pathway inhibitors, and cloned RLR and related genes into an expression plasmid.
