Engineered Human Reporter Jurkat T Lymphocytes
Jurkat cells are an immortalized cell line that was obtained from the peripheral blood of a boy with T-cell leukemia. This suspension cell line is easy to propagate and transfect. It has most often been used as a prototypical T-cell line to delineate the signaling pathways induced by the engagement of T-cell receptor (TCR) and to study the expression of various chemokine receptors susceptible to viral entry. The TCR signaling cascades result in the nuclear translocation of transcription factors, such as NFAT (nuclear factor of activated T cells), NF-κB (nuclear factor kappa-light-chain-enhancer of activated B cells), and IRFs (interferon regulatory factors) to induce the expression of target genes. Jurkat-derived cells are useful to screen for antibodies or small molecules mimicking or blocking the TCR stimulation or co-stimulation.
All our cell lines are extensively tested for viability, stability, biological activity, and absence of mycoplasma to ensure strong and reproducible results. Moreover, we provide detailed handling and experimental procedures for all cell lines, to minimize the need for optimization or troubleshooting by the end-user.
Jurkat cell line collection
- Transcription Factor Reporter Cells
- Cluster of Differentiation Reporter Cells
- Immune Checkpoint Reporter Cells
- T cell receptor reporter cells (CD4+, CD8+, or MAIT cells)
Key Features
- Verified overexpression of Fc-gamma receptors, co-stimulatory CD28, or immune checkpoints
- Stable expression of one or two reporter systems
- Quantifiable responses upon stimulation with target cells and/or agonist molecules
- Functionally tested and guaranteed mycoplasma-free
InvivoGen's Jurkat-Lucia™ Reporter Cells feature the inducible Lucia luciferase reporter gene. Jurkat-Dual™ Reporter Cells feature the inducible Lucia luciferase and SEAP (secreted embryonic alkaline phosphatase) reporter genes. Both reporter genes are placed under the control of either NFAT, NF-κB, or IRF transcription factors. The reporter activity is readily measurable in the cell culture supernatant when using QUANTI-Blue™, a SEAP detection reagent, or QUANTI-Luc™ 4 Lucia/Gaussia, a Lucia luciferase detection reagent.
