|Jurkat-Dual™ cells||Unit size||Cat. code||Docs||Qty||Price|
Human T lymphocytes - NF-kB/IRF reporter cells
3-7 x 10e6 cells
IRF-SEAP & NF-kB-Luc Reporter T lymphocytes
Jurkat-Dual™ cells were derived from the human T lymphocyte-based Jurkat cell line by stable integration of two inducible reporter constructs.
As a result, Jurkat-Dual™ cells allow the simultaneous study of the NF-κB pathway, by monitoring the activity of a secreted luciferase (Lucia), and the IRF pathway, by assessing the activity of SEAP.
Both reporter proteins are readily measurable in the cell culture supernatant when using QUANTI-Luc™, a luciferase detection reagent, and QUANTI-Blue™, a SEAP detection reagent.
Jurkat-Dual™ cells are resistant to the selectable markers Zeocin™ and blasticidin.
Jurkat-Dual™ cells induce the activation of NF-κB in response to TNF-α and T-lymphocyte mitogens, such as phytohemagglutinin and concanavalin A. They trigger the IRF pathway upon stimulation with type I IFNs and poly(I:C).Back to the top
These products are covered by a Limited Use License (See Terms and Conditions).Back to the top
- 1 vial of Jurkat-Dual™ cells (3-7 x 106 cells) in Freezing Medium
- 1 ml Normocin™ (50 mg/ml). Normocin™ is a formulation of three antibiotics active against mycoplasmas, bacteria and fungi
- 100 μl Zeocin™ (100 mg/ml)
- 100 μl Blasticidin (10 mg/ml)
- 1 pouch of QUANTI-Luc™
- 1 ml of QB reagent and 1 ml of QB buffer (sufficient to prepare 100 ml of QUANTI-Blue™ Solution, a SEAP detection reagent)
Shipped on dry ice (Europe, USA & Canada)Back to the top
Jurkat-Dual™ cells feature the Lucia luciferase gene, a secreted luciferase reporter gene, driven by an IFN-β minimal promoter fused to five copies of the NF-κB consensus transcriptional response element and three copies of the c-Rel binding site.
Jurkat-Dual™ cells also express a secreted embryonic alkaline phosphatase (SEAP) reporter gene under the control of an ISG54 minimal promoter in conjunction with five IFN-stimulated response elements.
As a result, Jurkat-Dual™ cells allow the simultaneous study of the NF-κB pathway, by monitoring the activity of Lucia luciferase, and the IRF pathway, by assessing the activity of SEAP.Back to the top
NF-κB/IRF dual response of Jurkat-Dual™ cells
Cells were incubated with 50 μg/ml phytohemagglutinin (PHA), 50 μg/ml concanavalin A (Con A), 104 EU/ml IFN-α, 200 ng/ml TNF-α or 10 μg/ml poly(I:C).
After 24h incubation, the levels of NF-κB-induced Lucia luciferase and IRF-induced SEAP were assessed from the cell culture supernatant using QUANTI-Luc™ or QUANTI-Blue™, respectively.