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Jurkat-Lucia™ NFAT Cells

Jurkat-Lucia™ NFAT Cells Unit size Cat. code Docs Qty Price
NFAT-Luc Reporter T lymphocytes
3-7 x 10e6 cells
jktl-nfat
+-
$1,035.00

NFAT-Luc Reporter T lymphocytes

Jurkat-LuciaNFAT cells were derived from the human T lymphocyte-based Jurkat cell line by stable integration of an NFAT-inducible Lucia reporter construct. The Lucia gene, which encodes a secreted coelenterazine-utilizing luciferase, is driven by an ISG54 minimal promoter fused to six copies of the NFAT consensus transcriptional response element.

Jurkat-LuciaNFAT cells induce the activation of NFAT in response to PMA and ionomycin, and T-lymphocyte mitogens, such as concanavalin A (ConA) and phytohemagglutinin.

Jurkat-Lucia™ NFAT cells can be used for the study of NFAT activation following stimuli treatments by monitoring Lucia luciferase activity. Levels of Lucia luciferase are readily measurable in the cell culture supernatant when using QUANTI-Luc™, a Lucia luciferase detection reagent.

Jurkat-LuciaNFAT cells are resistant to the selective antibiotic Zeocin™.


Jurkat-Lucia™ NFAT cells induced by PMA+ionomycin, or by T-lymphocyte mitogen concanavalin A (ConA).

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Specifications

Antibiotic resistance: Zeocin™

Guaranteed mycoplasma-free

These products are covered by a Limited Use License (see Terms and Conditions).

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Contents

  • 1 vial of Jurkat-Lucia™ NFAT cells (3-7 x 106 cells) in Freezing Medium
  • 1 ml Normocin™ (50 mg/ml). Normocin™ is a formulation of three antibiotics active against mycoplasmas, bacteria and fungi
  • 100 μl Zeocin™ (100 mg/ml)
  • 1 pouch of QUANTI-Luc™

Shipped on dry ice

IMPORTANT: Cells are shipped frozen. If cells are not frozen upon arrival, contact InvivoGen immediately.

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Description

Jurkat-Lucia-NFAT pathway

NFAT (nuclear factor of activated T cells) proteins are a family of transcription factors that are important in the development and function of the immune system. In T cells, NFAT proteins regulate thymocyte development, T cell activation, differentiation and self-tolerance. Their activation is induced by the engagement of the T cell receptor (TCR) that is coupled to the calcium-signaling pathway. Calcium binds calmodulin and activates calcineurin, which in turn dephosphorylates NFAT proteins inducing their nuclear translocation and activation, and the transcription of specific responsive genes.

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Details

Jurkat-Lucia-NFAT cells PMA ConA graph

 

Jurkat-Lucia™ NFAT cells induced by PMA+ionomycinor by T-lymphocyte mitogen concanavalin A (ConA).

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