InvivoGen provides a collection of control monoclonal antibodies (mAb) designed to help differentiate non-specific background from specific antibody signal.
In general, control mAbs should match the primary antibody's host, isotype and subclass, method of production, and working concentration.
The control mAb family comprises the variable region of an E.coli anti-β-galactosidase (β-Gal) mAb and the constant region of different native or engineered human, mouse or rat isotypes. These mAbs differ in their effector functions, such as complement-dependent cytotoxicity (CDC) and antibody-dependent cell-mediated cytotoxicity (ADCC).
For our bispecific antibodies, we provide negative control mAbs featuring two binding sites: β-Gal and hCD3 or β-Gal and hCD19.
InvivoGen offers the following control mAb isotypes:
Human Isotype Controls: hIgA2, hIgG1, hIgG1fut, hIgG1 (N298A), hIgG2, hIgG3, hIgG4 and hIgG4 (S228P)
Murine Isotype Controls: mIgG1, mIgG1e3, mIgG2a and mIgG2b
Rat Isotype Controls: rIgG1, rIgG2a and rIgG2b
Bispecific Antibody Controls: Negative controls for the bispecific antibody featuring two binding sites, either hCD3 or hCD19, linked to β-Gal
Our control mAbs are either produced in hybridoma cells or in Chinese hamster ovary (CHO) cells using recombinant technology. They can be used for blocking, neutralization, depletion or activation assays in vivo or in vitro, as well as for other applications, including immunofluorescence (IF), immunocytochemistry (ICC), immunoprecipitation (IP), and ELISA.