InvivoGen is a leader in the production of selection antibiotics.
Our state-of-the-art facilities allow us to produce large quantities of high quality, endotoxin-free antibiotics with purity levels exceeding 95%. As we manufacture our products, we are able to offer the best prices on the market.
Ready-to-use Cell Culture Tested Solutions - No weighing needed - Our antibiotics are available in solution filtered to sterility for customer convenience and validated for cell culture usage.
An Excellent Choice of Antibiotics for Selection in Both Mammalian cells and E. coli - Matches up to the antibiotic resistance genes carried by InvivoGen plasmids, built for selection in both mammalian and bacteria cells.
Frequently Asked Questions
Selective antibiotics general questions
Indeed, any selective antibiotics can be used together at the same time and in any combination.
We recommend not adding selective antibiotics until the cells have been passaged twice as cells are fragile during the initial culture procedure and the antibiotics may reduce cell viability. The resistance marker has to be expressed enough for the cells to acquire the resistance to these selective antibiotics.
For deactivation of all our selective antibiotics (Zeocin®, Hygromycin, G418, Phleomycin, Puromycin, and Blasticidin), we would recommend adding to the growth medium:
- - 5% NaOH (Sodium hydroxide)
- - 0.1% NaClO (Sodium hypochlorite)
You should then leave to react for 5 – 10 minutes and this should effectively deactivate all antibiotics in media.
Unfortunately we do not have data on the half-life of our selective antibiotics in cell culture medium. In house we prefer to add the selective antibiotics extemporaneously however if necessary you can supplement the medium with the antibiotic of your choice and keep at 4°C for no more than 1 week.
All of our selection antibiotics are shipped at room temperature. Upon receipt, they should be stored at 4°C or -20°C. Avoid repeated freeze-thaw cycles.
The expiry date is specified on the product label.
Once opened they remain stable for 1 year at -20°C providing you avoid repeated freeze-thaw cycles and they can be kept at 4°C for 3 months providing they are kept in sterile conditions.