Phleomycin

Selection antibiotic for bacteria and mammalian cells – Endotoxin-tested

Phleomycin is a selection antibiotic for mammalian and bacterial cells. It is sterile, cell culture tested, and available as a solution. This member of the bleomycin family is a glycopeptide antibiotic isolated from a mutant strain of Streptomyces verticillus. 

Phleomycin binds and intercalates DNA thus destroying the integrity of the double helix [1]. It is active against most bacteria, filamentous fungi, yeast, plant, and animal cells. 

Phleomycin resistance is conferred by the Sh ble gene from Streptoalloteichus hindustanus, which encodes a protein that binds to phleomycin, inhibiting its DNA cleavage activity.

The use of phleomycin is recommended for cells that are poorly sensitive to Zeocin®, such as filamentous fungi and some yeasts. Typically, phleomycin is used at a concentration of 25 to 150 µg/ml for filamentous fungi, and 10 µg/ml for yeasts.

Key features

• Produced in InvivoGen's facilities
• Each lot thoroughly tested to ensure lot-to-lot reproducibility
• Potency validated in Zeocin®-sensitive and Zeocin®-resistant mammalian cell lines

• Absence of long-term effects confirmed in Zeocin®-resistant cells

   

Reference:

1. Wilson JB, et al., 2010. Several tetratricopeptide repeat (TPR) motifs of FANCG are required for assembly of the BRCA2/D1-D2-G-X3 complex, FANCD2 monoubiquitylation and phleomycin resistance. Mutat Res. 689(1-2):12-20.