Hygromycin B Gold

Hygromycin B Gold (solution) Unit size Cat. code Docs Qty Price
Selective antibiotic for the hph gene
1 g (10 x 1 ml)
5 g (1 x 50 ml)

Selective antibiotic | Hygromycin B Gold™ | InvivoGen

Hygromycin B Gold: an ultrapure Hygromycin B.

Hygromycin B is an aminoglycoside antibiotic produced by Streptomyces hygroscopicus. It kills bacteria, fungi and higher eukaryotic cells by inhibiting protein synthesis.

It has been reported to interfere with translocation [1] and to cause mistranslation at the 70S ribosome [2].

Resistance to Hygromycin B

Hygromycin B is used as a selective agent in molecular genetics experiments on a wide variety of eukaryotic and prokaryotic species [3-5].

The hph gene confers hygromycin-resistance to cells expressing it and many vectors carrying the hph gene are available within the scientific community.

Typically, mammalian cells are sensitive to Hygromycin B concentrations of 50-200 µg/ml, and bacteria to 50-100 µg/ml.


Protocol to facilitate the transition from using Hygromycin B to Hygromycin B Gold.



1. Cabanas M. et al., 1978. Dual interference of Hygromycin B with ribosomal translocation and with Aminoacyl-tRNA recognition. Eur. J. Biochem. 87:21‑7.
2. Gonzales, A. et al., 1978. Studies on the mode of action of hygromycin B, an inhibitor of translocation in eukaryotes. Biochem Biophys Acta 521:459‑69.
3. Gritz L. & Davies J., 1983. Plasmid-encoded hygromycin B resistance: the sequence of hygromycin B phosphotransferase gene and its expression in Escherichia coli and Saccharomyces cerevisiae. Gene 25:179-88.
4. Cullen D. et al., 1987. Transformation of Aspergillus nidulans with the hygromycin-resistance gene, hph. Gene 57:21-6.
5. Santerre R. et al., 1984. Expression of prokaryotic genes for hygromycin B and G418 resistance as dominant-selection markers in mouse L cells. Gene 30:147-56.


Hygromycin B Gold™ by InvivoGen
Hygromycin B Gold™ by InvivoGen
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Product concentration: 100 mg/ml solution

CAS number: 31282-04-9

Quality Control: Each lot is thoroughly tested to ensure the absence of lot-to-lot variation.

Purity: ≥ 90% (HPLC)

Endotoxin level: < 0.5 EU/mg

Physicochemical characterization: pH, appearance

Cell culture tested: potency validated in hygromycin‑sensitive and hygromycin-resistant mammalian cell lines

Non‑cytotoxicity of trace contaminants: absence of long‑term effects confirmed in hygromycin‑resistant cells

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Hygromycin B Gold (previously named HygroGold™) is supplied as a sterile filtered yellow solution at 100 mg/ml in HEPES buffer.

This product is available in two pack sizes:

  • ant-hg-1: 10 x 1 ml (1 g)
  • ant-hg-5: 1 x 50 ml (5 g)

Hygromycin B Gold is shipped at room temperature.

Upon receipt, it should be stored at 4°C or -20˚C.

SPECIAL HANDLING : Hygromycin B is a hazardous compound. Avoid contact with skin and eyes.

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The working concentrations of Hygromycin B Gold for mammalian cell lines vary from 50 to 200 μg/ml, in a few cases up to 500 μg/ml.
If you notice unusually high cytotoxicity when switching to Hygromycin B Gold, you may need to adjust your working concentrations.
Suggested concentrations of Hygromycin B Gold for selection in some examples of mammalian cells are listed below.

Cell line Species Tissue Medium Hygromycin B Equivalent concentrations
Hygromycin B
(ant-hm-1, ant‑hm-5)
Hygromycin B Gold
(ant-hg-1, ant-hg-5)
B16  Mouse Melanoma RPMI 100-200 µg/ml 200 µg/ml 160 µg/ml
CHO Hamster Ovary MEM 100-500 µg/ml 500 µg/ml 400 µg/ml
HeLa Human Cervix DMEM 100-200 µg/ml 200 µg/ml 160 µg/ml
HEK293 Human Kidney DMEM 50-400 µg/ml 500 µg/ml 320 µg/ml
Raji Human Lymphoma RPMI 125-300 µg/ml 300 µg/ml 240 µg/ml
THP-1 Human Monocytes RPMI 200-400 µg/ml 500 µg/ml 320 µg/ml


Formula: C20H37N3O13, HCl

Molecular weight: 563.5


Hygromycin chemical structure

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