|G418 (solution)||Unit size||Cat. code||Docs||Qty||Price|
Selective antibiotic for the neo gene
1 g (10 x 1ml)
2 g (20 x 1 ml)
5 g (1 x 50 ml)
G418 (Geneticin) : selection antibiotic, cell culture tested, sterile reagent
G418 also known as G-418, G418 sulfate or Geneticin is an aminoglycoside antibiotic produced by Micromonospora rhodorangea.
G418 blocks polypeptide synthesis by inhibiting the elongation step in both prokaryotic and eukaryotic cells.
Selection in mammalian cells is usually achieved in 3 to 7 days with concentrations ranging from 400 to 1000 µg/ml.
Cells that are dividing are affected more rapidly than non-dividing cells.Back to the top
Product concentration: 100 mg/ml
CAS number: 108321-42-2
Quality Control: Each lot is thoroughly tested to ensure the absence of lot-to-lot variation.
Purity: ≥ 90% (HPLC)
Endotoxin level: < 0.5 EU/mg
Physicochemical characterization: pH, appearance
Cell-culture tested: potency validated in G418-sensitive and G418-resistant mammalian cell lines
Non-cytotoxicity of trace contaminants: absence of long-term effects confirmed in G418-resistant cells
Formula: C20H40N4O10. 2H2SO4
Molecular weight: 692.7
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G418 is supplied as a sterile filtered solution at 100 mg/ml in HEPES buffer.
This product is available in three pack sizes:
- ant-gn-1: 10 x 1 ml (1 g)
- ant-gn-2: 20 x 1 ml (2 g)
- ant-gn-5: 1 x 50 ml (5 g)
G418 is shipped at room temperature.
Upon receipt it should be stored at 4°C.Back to the top
G418 is normally used at a concentration of 400 μg/ml. However, the optimal concentration needs to be determined for your cells.
Suggested concentrations of G418 for selection in some examples of mammalian cells are listed below:
|B16 (Mouse melanocytes)||RPMI||400-1000 μg/ml||1, 2|
CHO (Chinese hamster ovarian cells)
|HeLa (Human uterine cells)||DMEM||200-400 μg/ml||5, 6|
|HEK293 (Human embryonic kidney cells)||DMEM||200-500 μg/ml||7, 8|
|THP-1 (Human monocytes)||RPMI||250 μg/ml||9, 10|
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