G418 (Geneticin)
| G418 (solution) | Unit size | Cat. code | Docs | Qty | Price |
|---|---|---|---|---|---|
Selective antibiotic for the neo gene |
1 g (10 x 1ml) 5 g (1 x 50 ml) |
ant-gn-1 |
G418 (Geneticin) : selection antibiotic, cell culture tested, sterile reagent
- What is G148 ?
- G418, also known as G418 sulfate and Geneticin, is an aminoglycoside antibiotic similar in structure to gentamicin B1, produced by Micromonospora rhodorangea.
- G418 blocks polypeptide synthesis by inhibiting the elongation step in both prokaryotic and eukaryotic cells.
- Resistance to G418 is conferred by the Neomycin resistance gene (neo) from Tn5 encoding an aminoglycoside 3'-phosphotransferase, APH 3' II.
- Selection in mammalian cells is usually achieved in 3 to 7 days with concentrations ranging from 400 to 1000 µg/ml.
Cells that are dividing are affected more rapidly than non-dividing cells.
Specifications
Product concentration: 100 mg/ml
CAS number: 108321-42-2
Quality Control: Each lot is thoroughly tested to ensure the absence of lot-to-lot variation.
Purity: ≥ 90% (HPLC)
Endotoxin level: < 0.5 EU/mg
Physicochemical characterization: pH, appearance
Cell-culture tested: potency validated in G418-sensitive and G418-resistant mammalian cell lines
Non-cytotoxicity of trace contaminants: absence of long-term effects confirmed in G418-resistant cells
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G418 is supplied as a sterile filtered solution at 100 mg/ml in HEPES buffer.
This product is available in two pack sizes:
- ant-gn-1: 10 x 1 ml (1 g)
- ant-gn-5: 1 x 50 ml (5 g)
G418 is shipped at room temperature.
Upon receipt it should be stored at 4°C.
