Understanding InvivoGen’s Product Grades
Certain InvivoGen products are assigned a specific quality grade to indicate their suitability for defined research applications. These grades reflect dedicated manufacturing processes, special buffer formulations, and quality control testing adapted to the primary intended use.
However, this designation is not restrictive, and products may still be used in other experimental settings depending on the protocol, with simple adjustments such as switching to an appropriate buffer. Please don’t hesitate to contact our Tech Support.
This guide helps you quickly identify the grade that best fits your experimental needs, from in vitro screening to pre-clinical in vivo studies. Below is the comparison table outlining the main features and quality control tests associated with each InvivoGen grade to help you select the most appropriate option for your study.
| Feature / Test | VacciGrade™ | EndoFit™ | InvivoFit™ | UltraPure | InvitroFit™ |
|---|---|---|---|---|---|
| Type of product | Vaccine adjuvants | Ovalbumin (OVA) | Antibodies | PRR ligands (TLR4, TLR5, NOD1/2) | Inhibitors |
| Developed for | In vivo studies | In vivo studies | In vivo studies | In vitro studies | In vitro studies |
| Suited for | In vivo & in vitro studies | In vivo & in vitro studies | In vivo & in vitro studies | In vitro & in vivo studies | In vitro & in vivo studies |
| Functional activity testing in vitro | ✓* | ✗ | ✓ | ✓ | ✓ |
| Purity > 95% | ✓* | ✓ | ✓ | ✓* | ✓* |
| HEK-Blue™ TLR2 assay | ✓ | ✓ | ✓ | ✓ | ✓ |
| HEK-Blue™ TLR4 assay | ✓ | ✓ | ✓ | ✓ | ✓ |
| LAL assay (endotoxins) | ✓ | ✓ | ✓ | ✓* | ✗ |
| Filter-sterilized (0.22 µm) | ✓* | ✓ | ✓ | ✗ | ✗ |
| Clean-room aseptic filling | ✓* | ✓ | ✓ | ✗ | ✗ |
| Specific buffer formulation | ✓ | ✓ | ✓ | ✗ | ✗ |
| Sterility test (pharmacopeia-based) | ✓ | ✓ | ✗ | ✗ | ✗ |
| Additional purification for ligand specificity | NA | NA | NA | ✓ | NA |
Legend: * = Depends on product (see technical data sheet) || ✓ = Performed || ✗ = Not performed || NA = Not applicable
Each grade explained in detail
VacciGrade™
Includes: Vaccine adjuvants (vehicles, immunostimulants, antigen-adjuvant conjugates)
Developed for: Pre-clinical in vivo studies | Suitable for: In vivo and in vitro studies
Description: VacciGrade™ is a high-quality and highly pure pre-clinical grade developed for in vivo applications of vaccine adjuvants where sterility is critical. Products are guaranteed sterile, filter-sterilized (0.22 µm), filled under strict aseptic conditions in a clean-room environment, and are formulated and provided either in dedicated buffers or in physiological water for vaccine adjuvant purposes, depending on the product.
Quality control: Each lot is tested for sterility based on the European Pharmacopoeia assay (2.6.1) and guaranteed free of unwanted bacterial contaminants as confirmed using a LAL assay as well as HEK-Blue™ TLR2 and HEK-Blue™ TLR4 cellular assays. Functional activity may be validated using established or proprietary cellular models.
EndoFit™
Includes: Ovalbumin (OVA)
Developed for: Pre-clinical in vivo studies | Suitable for: In vivo and in vitro studies
Description: EndoFit™ is a high-quality and highly pure (≥98%) pre-clinical grade developed for in vivo applications of ovalbumin (OVA). Commercially available OVA is often contaminated with endotoxins which may alter the results obtained in vivo. InvivoGen's product is guaranteed sterile, filled under strict aseptic conditions in a clean-room environment, and is formulated without salts. It is provided with physiological water for vaccine adjuvant purposes.
Quality control: Each lot is tested for sterility based on the European Pharmacopoeia assay (2.6.1) and guaranteed free of unwanted bacterial contaminants as confirmed using a LAL assay as well as HEK-Blue™ TLR2 and HEK-Blue™ TLR4 cellular assays.
InvivoFit™
Includes: Monoclonal, recombinant mouse antibodies
Developed for: In vivo studies | Suitable for: In vivo and in vitro studies
Description: InvivoFit™ is a high-quality grade for antibodies specifically designed for in vivo applications. Products are highly pure (≥95%), formulated in a buffer adapted for in vivo use, filter-sterilized (0.22 µm), and filled under strict aseptic conditions in a clean-room environment.
Quality control: Each lot is tested for bacterial contaminants, including endotoxins and lipoproteins, using a LAL assay as well as HEK-Blue™ TLR2 and HEK-Blue™ TLR4 cellular reporter assays. This ensures suitability for sensitive in vivo experiments. Functional activity is systematically validated using established or proprietary cellular models, ensuring reliability and reproducibility.
InvitroFit™
Includes: Inhibitors
Developed for: In vitro assays | Suitable for: In vitro and in vivo studies
Description: InvitroFit™ is a high-quality grade optimized for in vitro research applications of inhibitors. Products are highly pure and manufactured to ensure consistent performance in cellular and biochemical assays.
Quality control: Each lot is confirmed to be free of bacterial contamination using HEK-Blue™ TLR2 and HEK-Blue™ TLR4 cellular assays. Functional activity is systematically validated using established or proprietary cellular models.
Ultrapure
Includes: PRR ligands (TLR4, TLR5, and NOD1/2)
Developed for: In vitro assays | Suitable for: In vitro and in vivo studies
Description: Ultrapure products are derived from Gram-positive and Gram-negative bacteria. They undergo additional purification steps to selectively isolate the desired ligand while minimizing the presence of other bacterial components.
Quality control: Each lot undergoes dedicated purification and quality control processes to ensure a high level of purity. Endotoxin levels are assessed using a LAL assay or HEK-Blue™ TLR2 and HEK-Blue™ TLR4 cellular reporter assays ensuring exclusive TLR4, TLR5, or NOD1/2 activation.
