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2'3'-cGAMP VacciGrade™

2’3’-cGAMP VacciGrade™ Unit size Cat. code Docs Qty Price
Mammalian (non-canonical) CDN - Cyclic [G(2’,5’)pA(3’,5’)p]
1 mg
vac-nacga23
+-
$643.00

STING-based vaccine adjuvant - Th1/Th2 response

2’3’-cGAMP VacciGrade™ is a preclinical grade preparation of the cyclic dinucleotide 2’3’-cGAMP (cyclic [G(2’,5’)pA(3’,5’)p]), a cyclic dinucleotide (CDN) produced in mammalian cells by cGAS (cGAMP synthase) in response to double-stranded DNA in the cytoplasm.
2’3’-cGAMP is also referred to as “noncanonical” cGAMP due to the presence of the atypical 2’-5’ phosphodiester linkage between the guanosine and the adenosine. Structural and functional studies revealed that noncanonical 2’3’-cGAMP is distinct from the canonical 3’3’-cGAMP produced by bacteria [1, 2].

CDNs are a relatively new class of adjuvants that have been shown to increase vaccine potency [3]. CDNs activate innate immunity by directly binding the endoplasmic reticulum-resident receptor STING (stimulator of interferon genes), leading to the expression of interferon-β (IFN-β) and nuclear factor-κB (NF-κB) dependent inflammatory cytokines.

2′3′-cGAMP is an effective adjuvant that boosts the production of antigen-specific antibodies and T cell responses in mice [4].

  • 2’3’-cGAMP VacciGrade™ is prepared under strict aseptic conditions and is guaranteed sterile
  • 2’3’-cGAMP VacciGrade™ is tested for the presence of endotoxins

 

Read our review on STING: Deciphering the STING Paradox

 

2’3’-cGAMP VacciGrade™ is for research use only, not for use in humans

 

References:

1. Diner E. et al., 2013. The Innate Immune DNA Sensor cGAS Produces a Noncanonical Cyclic Dinucleotide that Activates Human STING. Cell Rep. 3(5):1355-61.
2. Gao P. et al., 2013. Cyclic [G(2',5')pA(3',5')p] is the metazoan second messenger produced by DNA-activated cyclic GMP-AMP synthase. Cell. 153(5):1094-107.
3. Dubensky TW. et al., 2013. Rationale, progress and development of vaccines utilizing STING-activating cyclic dinucleotide adjuvants. Therapeutic Advances in Vaccines 1(4): 131-143.
4. Li XD. et al., 2013. Pivotal roles of cGAS-cGAMP signaling in antiviral defense and immune adjuvant effects. Science. 341(6152):1390-4.

Figures

IRF INDUCTION (Lucia luciferase reporter)
IRF INDUCTION (Lucia luciferase reporter)

THP1-Dual™ cells were stimulated for 24 hours with the STING ligands as shown (all at 10 μg/ml). IRF induction was determined by measuring the relative light units (RLUs) in a luminometer using QUANTI‑Luc™, a Lucia luciferase detection reagent.


THP1-Dual™ cells were stimulated for 24 hours with the STING ligands as shown (all at 10 μg/ml). NF‑kB induction was determined using QUANTI‑Blue™, a SEAP detection reagent, and by reading the optical density (OD) at 655 nm. Non-induced cells (NI) have been included as a negative control.

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Specifications

Description: STING agonist VacciGrade™

Synonym: Cyclic [G(2’,5’)pA(3’,5’)p], cyclic GMP-AMP; c-GpAp sodium salt

CAS number: 1441190-66-4

Formula: C20H22N10O13P2 .2Na

Molecular weight: 718.38

Purity: ≥ 95% by LC/MS

Solubility: 50 mg/ml in physiological water

Quality: Sterile

Endotoxin Level: Lower than 0.005 EU/μg

Biological activity: Assessed by measuring induction of the interferon pathway in THP1-Blue™ ISG cells

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Contents

  • 1 mg (2 x 500 µg) lyophilized 2’3’-cGAMP VacciGrade™
  • 10 ml sterile endotoxin-free physiological water (NaCl 0.9%)

2’3’-cGAMP VacciGrade™ is shipped at room temperature
2’3’-cGAMP VacciGrade™should be stored at -20°C.

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Citations

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