2'3'-cGAMP VacciGrade™ - STING-based Adjuvant

Mammalian (non-canonical) CDN - Cyclic [G(2’,5’)pA(3’,5’)p] | Th1/Th2 response

ABOUT

STING-based vaccine adjuvant - Th1/Th2 response

2’3’-cGAMP VacciGrade™ is a preclinical grade preparation of the cyclic dinucleotide 2’3’-cGAMP (cyclic [G(2’,5’)pA(3’,5’)p]), a cyclic dinucleotide (CDN) produced in mammalian cells by cGAS (cGAMP synthase) in response to double-stranded DNA in the cytoplasm.
2’3’-cGAMP is also referred to as “noncanonical” cGAMP due to the presence of the atypical 2’-5’ phosphodiester linkage between the guanosine and the adenosine. Structural and functional studies revealed that noncanonical 2’3’-cGAMP is distinct from the canonical 3’3’-cGAMP produced by bacteria [1, 2].

CDNs have been shown to increase vaccine potency [3]. CDNs activate innate immunity by directly binding the endoplasmic reticulum-resident receptor STING (stimulator of interferon genes), leading to the expression of interferon-β (IFN-β) and nuclear factor-κB (NF-κB) dependent inflammatory cytokines.

2′3′-cGAMP is an effective adjuvant that boosts the production of antigen-specific antibodies and T cell responses in mice [4].

2’3’-cGAMP VacciGrade™ is a high-quality pre-clinical grade.

 

Read our review Read our review on STING: Deciphering the STING Paradox

 

References:

1. Diner E. et al., 2013. The Innate Immune DNA Sensor cGAS Produces a Noncanonical Cyclic Dinucleotide that Activates Human STING. Cell Rep. 3(5):1355-61.
2. Gao P. et al., 2013. Cyclic [G(2',5')pA(3',5')p] is the metazoan second messenger produced by DNA-activated cyclic GMP-AMP synthase. Cell. 153(5):1094-107.
3. Dubensky TW. et al., 2013. Rationale, progress, and development of vaccines utilizing STING-activating cyclic dinucleotide adjuvants. Therapeutic Advances in Vaccines 1(4): 131-143.
4. Li XD. et al., 2013. Pivotal roles of cGAS-cGAMP signaling in antiviral defense and immune adjuvant effects. Science. 341(6152):1390-4.

All products are for research use only, and not for human or veterinary use.

VacciGrade™

VacciGrade™ is a high-quality pre-clinical grade, suitable for in vivo studies. VacciGrade™ products are filter-sterilized (0.2 µm) and filled under strict aseptic conditions in a clean room. The absence of  bacterial contamination is assessed by a sterility test using a pharmacopeia-derived assay.

SPECIFICATIONS

Specifications

Source
Synthetic
Synonyms
Cyclic [G(2’,5’)pA(3’,5’)p]
c-GpAp sodium salt
Cyclic GMP-AMP
CAS #1441190-66-4 (free acid)
Target

STING

CAS number
2734858-36-5
Chemical formula

C20H22N10O13P2 •2Na

Sequence

cyclic [G(2’,5’)pA(3’,5’)p]

Molecular weight
718.38 g/mol
Purity
≥ 95% by LC/MS
Solubility

50 mg/ml in physiological water

Appearance (form)
Lyophilized
Reconstitution buffer
Endotoxin-free water (provided)
Sterility

Sterility guaranteed

Endotoxin

< 5 EU/mg (measurement by kinetic chromogenic LAL assay)

Tested applications

Induction of the interferon pathway in cellular assays

Quality control

Each lot is functionally tested and validated.

CONTENTS

Contents

  • Product: 
    2’3’-cGAMP VacciGrade™
  • Cat code: 
    vac-nacga23
  • Quantity: 
    1 mg
Includes:

10 ml sterile endotoxin-free physiological water (NaCl 0.9%)

Shipping & Storage

  • Shipping method:  Room temperature
  • Storage:

    • -20°C
    Stability: -20°C for up to 6 months

    Caution:

    • Avoid repeated freeze-thaw cycles

Details

Structure of 2'3'-cGAMP VacciGrade™

DOCUMENTS

Documents

2’3’-cGAMP VacciGrade™

Technical Data Sheet

Safety Data Sheet

Validation Data Sheet

Certificate of analysis

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CUSTOMER SERVICE & TECHNICAL SUPPORT

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