Flagellin FliC VacciGrade™

Recombinant flagellin from S. typhimurium

Bacterial flagellin, a TLR5 ligand, is a potent T-cell antigen and has potential as a vaccine adjuvant. Unlike other TLR agonists, flagellin tends to produce mixed Th1 and Th2 responses rather than strongly Th1 responses.

Flagellin Flic VacciGrade™ is suitable for preclinical studies. It is prepared under strict aseptic conditions. It is guaranteed sterile and thoroughly tested for the presence of endotoxins.

Flagellin Flic VacciGrade™ is for research use only, not for use in humans.

Specifications

Specificity: Th1/Th2 response

Working concentration: 1 -10 μg/mouse

Solubility: 500 µg/ml in physiological water

Quality control :

• Flagellin FliC is VacciGrade™ (preclinical grade).
• It is prepared under strict aseptic conditions and is tested for the presence of endotoxins.
• Flagellin FliC VacciGrade™ is guaranteed sterile.
• endotoxin level : <0.005  EU/mg  (measurement  by  kinetic  chromogenic  LAL  assay)

Contents

• 50 µg of sterile lyophilized Flagellin FliC VacciGrade™
• 10 ml sterile endotoxin-free physiological water (NaCl 0.9%)

Flagellin FliC VacciGrade™ is shipped at room temperature.

Flagellin FliC VacciGrade™ should be stored at -20°C.

Lyophilized product is stable 1 year at -20°C when properly stored.

Description

Flagellin FliC is a recombinant flagellin protein encoded by the fliC gene from Salmonella typhimurium. Bacterial flagellin, a TLR5 ligand, is a potent T-cell antigen and has potential as a vaccine adjuvant.

Unlike other TLR agonists, flagellin tends to produce mixed Th1 and Th2 responses rather than strong Th1 responses [1].

It has been demonstrated that flagellin can act as a potent adjuvant in flu vaccines [2, 3].

Furthermore, flagellin can also signal through the NLRC4 inflammasome [4], although it is not known whether this pathway contributes to the adjuvant activity of flagellin.

1. Huleatt J. et al., 2007. Vaccination with recombinant fusion proteins incorporating Toll-like receptor ligands induces rapid cellular and humoral immunity. Vaccine 25(4): 763-75.
2. Mbow ML. et al., 2010. New adjuvants for human vaccines. Curr Opin Immunol. 22(3):411-6.
3. Skountzou I. et al., 2010. Salmonella flagellins are potent adjuvants for intranasally administered whole inactivated influenza vaccine. Vaccine 28(4): 4103-12.
4. Miao EA. & Warren SE., 2010. Innate immune detection of bacterial virulence factors via the NLRC4 inflammasome. J Clin Immunol. 30(4): 502-6