ABOUT
Recombinant flagellin from S. typhimurium
Flagellin FliC VacciGrade™ is a recombinant flagellin protein encoded by the fliC gene from the Gram-negative bacteria Salmonella typhimurium. This protein is produced in CHO cells and purified by affinity chromatography. It is intended for use in vaccine adjuvant research.
Bacterial flagellin, a TLR5 ligand, is a potent T cell antigen and has potential as a vaccine adjuvant. Unlike other TLR agonists, flagellin tends to produce mixed Th1 and Th2 responses rather than strongly Th1 responses.
Flagellin FliC VacciGrade™ is a high-quality pre-clinical grade.
InvivoGen also offers recombinant Flagellin FliC in a standard grade for in vitro experiments.
All products are for internal research use only, and not for human or veterinary use.
VacciGrade™
VacciGrade™ is InvivoGen’s highest-quality grade, suitable for in vivo studies. Products are filter-sterilized (0.22 µm), aseptically filled in a cleanroom, and their sterility is confirmed by a pharmacopeia-based assay.
SPECIFICATIONS
Specifications
TLR5
1 -10 μg/mouse
500 µg/ml in physiological water
0.22 µm filtration, Sterility guaranteed
≤ 0.005 EU/µg (measurement by kinetic chromogenic LAL assay)
TLR5 activation in cellular assays
Vaccine adjuvant research
TLR5 activation
Each lot is functionally tested and validated using cellular assays.
Do not vortex.
The absence of bacterial contamination (e.g. lipoproteins and endotoxins) has been confirmed using HEK-Blue-Lucia™ TLR2 and HEK‑Blue-Lucia™ TLR4 cells.
CONTENTS
Contents
-
Product:Flagellin FliC VacciGrade™
-
Cat code:vac-fla
-
Quantity:50 µg
10 ml sterile endotoxin-free physiological water (NaCl 0.9%)
Shipping & Storage
- Shipping method: Room temperature
- -20°C
- Avoid repeated freeze-thaw cycles
Storage:
Caution:
Details
Flagellin FliC is a recombinant flagellin protein encoded by the fliC gene from Salmonella typhimurium. Bacterial flagellin, a TLR5 ligand, is a potent T-cell antigen and has potential as a vaccine adjuvant.
Unlike other TLR agonists, flagellin tends to produce mixed Th1 and Th2 responses rather than strong Th1 responses [1].
It has been demonstrated that flagellin can act as a potent adjuvant in flu vaccines [2, 3].
Furthermore, flagellin can also signal through the NLRC4 inflammasome [4], although it is not known whether this pathway contributes to the adjuvant activity of flagellin.
1. Huleatt J. et al., 2007. Vaccination with recombinant fusion proteins incorporating Toll-like receptor ligands induces rapid cellular and humoral immunity. Vaccine 25(4): 763-75.
2. Mbow ML. et al., 2010. New adjuvants for human vaccines. Curr Opin Immunol. 22(3):411-6.
3. Skountzou I. et al., 2010. Salmonella flagellins are potent adjuvants for intranasally administered whole inactivated influenza vaccine. Vaccine 28(4): 4103-12.
4. Miao EA. & Warren SE., 2010. Innate immune detection of bacterial virulence factors via the NLRC4 inflammasome. J Clin Immunol. 30(4): 502-6
DOCUMENTS
Documents
Technical Data Sheet
Safety Data Sheet
Certificate of analysis
Need a CoA ?
Powered by Bioz
