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Monophosphoryl Lipid A from S. minnesota R595 - TLR4-based adjuvant
Monophosphoryl Lipid A from Salmonella minnesota R595 - TLR4-based adjuvant
The TLR4 agonist, MPLA (monophosphoryl lipid A) is a derivative of lipid A from Salmonella minnesota R595 lipopolysaccharide (LPS or endotoxin).
MPLA is considerably less toxic than LPS whilst maintaining immunostimulatory activity.
When tested in animal models as a vaccine adjuvant, MPLA induces a strong Th1 response.
MPLA-SM VacciGrade™ is a high-quality pre-clinical grade.
MPLA-SM VacciGrade™ is for research use only, and not for human or veterinary use.
Species: Salmonella enterica serovar minnesota mutant R595
Description: TLR4 agonist VacciGrade™
Polarization of adaptive immune response: Th1 response
Working concentration: 2- 20 μg/mouse
Solubility: 1 mg/ml in DMSO
- Sterility guaranteed
- The presence of other bacterial components is controlled using HEK-Blue™ TLR2 cells
- 1 mg of lyophilized MPLA-SM VacciGrade™
- 10 ml sterile endotoxin-free physiological water (NaCl 0.9%)
MPLA-SM VacciGrade™ is shipped at room temperature.
Upon receipt it should be stored at -20°C.
Product is stable 1 year when properly stored.
Upon resuspension, prepare aliquots of MPLA-SM VacciGrade™ and store at -20°C. Product is stable 6 months when properly stored.
Avoid repeated freeze-thaw cycles.Back to the top
VacciGrade™ is a high-quality pre-clinical grade. VacciGrade™ products are filter-sterilized (0.2 µm) and filled under strict aseptic conditions in a clean room*. The absence of bacterial contamination is assessed by a sterility test using a pharmacopeia-derived assay. The level of bacterial contaminants (endotoxins and lipoproteins) in each lot is verified using a LAL assay and/or a TLR2 and TLR4 reporter assay.
*Except for LPS VacciGrade™, which is prepared in a PSM dedicated to LPS.
Monophosphoryl lipid A (MPLA) is a low-toxicity derivative of the lipid A region of lipopolysaccharide (LPS), that retains the immunologically active lipid A portion of the parent molecule . While the toxicity associated with LPS prohibits its potential clinical use, MPLA is being developed as a vaccine adjuvant .
Both LPS and MPLA are TLR4 agonists, but they signal through different adaptors, MyD88 and TRIF, respectively. The reduced toxicity of MPLA is attributed to the preferential recruitment of TRIF upon TLR4 activation, resulting in decreased induction of inflammatory cytokines .
MPLA has been tested as an adjuvant in mice and reported to induce a strong Th1 response [4-6]. Although the mechanism of action of MPLA has not been fully eludicated, it has been suggested that MPLA improves vaccine immunogenicity by enhancing antigen presenting cell maturation .
1. Okemoto K. et al., 2006. A potent adjuvant monophosphoryl lipid A triggers various immune responses, but not secretion of IL-1beta or activation of caspase-1. J Immunol. 176(2):1203-8.
2. Casella CR. et al., 2008. Putting endotoxin to work for us: monophosphoryl lipid A as a safe and effective vaccine adjuvant. Cell Mol Life Sci. 65(20):3231-40.
3. Mata-Haro V. et al., 2007. The vaccine adjuvant monophosphoryl lipid A as a TRIF-biased agonist of TLR4. Science. 316(5831):1628-32.
4. Fransen F. et al., 2007. Agonists of Toll-like receptors 3, 4, 7, and 9 are candidates for use as adjuvants in an outer membrane vaccine against Neisseria meningitidis serogroup. Infect Immun. 75(12) :5939-46.
5. Rhee eG. et al., 2010. TLR4 Ligands Augment Antigen-Specific CD8+ T Lymphocyte Responses Elicited by a Viral Vaccine Vector. J. Virol. 84: 10413 - 10419.
6. Didierlaurent A. et al., 2009.AS04, an aluminum salt- and TLR4 agonist-based adjuvant system, induces a transient localized innate immune response leading to enhanced adaptive immunity. J Immunol 183(10): 6186-97.