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c-di-AMP VacciGrade™

c-di-AMP VacciGrade™ Unit size Cat. code Docs Qty Price
Cyclic diadenylate monophosphate - STING agonist
1 mg
vac-nacda
+-
$369.00

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Cyclic diadenylate monophosphate - STING agonist

Cyclic diadenylate monophosphate (c-di-AMP) is a cyclic dinucleotide (CDN) produced by bacteria in which it functions as an essential second messenger [1]. It coordinates bacterial growth, cell wall stability, and responses to stress and plays a crucial role in the establishment of bacterial infection.

CDNs have been shown to increase vaccine potency [2]. CDNs activate innate immunity by directly binding the endoplasmic reticulum-resident receptor STING (stimulator of interferon genes), activating a signaling pathway that induces the expression of interferon-β (IFN-β) and also nuclear factor-κB (NF-κB) dependent inflammatory cytokines [3, 4].

c-di-AMP exerts strong adjuvant activities when delivered by the mucosal route [5, 6].

 

c-di-AMP VacciGrade™ is a high-quality pre-clinical grade.

c-di-AMP VacciGrade™ ​ is for research use only, and not for human or veterinary use.

 

References:

1. Witte CE. et al., 2013. Cyclic di-AMP is critical for Listeria monocytogenes growth, cell wall homeostasis, and establishment of infection. MBio. 4(3):e00282-13.
2. Dubensky TW. et al., 2013. Rationale, progress and development of vaccines utilizing STING-activating cyclic dinucleotide adjuvants. Therapeutic Advances in Vaccines 1(4): 131-143.
3. Jin L. et al., 2011. MPYS is required for IFN response factor 3 activation and type I IFN production in the response of cultured phagocytes to bacterial second messengers cyclic-di-AMP and cyclic-di-GMP. J Immunol. 187(5):2595-601.
4. Burdette DL. et al., 2011. STING is a direct innate immune sensor of cyclic di-GMP. Nature. 478(7370):515-8.
5. Ebensen T. et al., 2011. Bis-(3',5')-cyclic dimeric adenosine monophosphate: strong Th1/Th2/Th17 promoting mucosal adjuvant.Vaccine. 29(32):5210-20.
6. Škrnjug I. et al., 2014. The mucosal adjuvant cyclic di-AMP exerts immune stimulatory effects on dendritic cells and macrophages. PLoS One. 9(4):e95728.

 

Figures

IRF INDUCTION (Lucia luciferase reporter)
IRF INDUCTION (Lucia luciferase reporter)

THP1-Dual™ cells were stimulated for 24 hours with the STING ligands as shown (all at 10 μg/ml). IRF induction was determined by measuring the relative light units (RLUs) in a luminometer using QUANTI‑Luc™, a Lucia luciferase detection reagent.

NF-kB INDUCTION (SEAP reporter)
NF-kB INDUCTION (SEAP reporter)

THP1-Dual™ cells were stimulated for 24 hours with the STING ligands as shown (all at 10 μg/ml). NF‑kB induction was determined using QUANTI‑Blue™, a SEAP detection reagent, and by reading the optical density (OD) at 655 nm. Non-induced cells (NI) have been included as a negative control.

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Specifications

Description: STING agonist VacciGrade™

Synonym: Cyclic di-adenylate monophosphate, c-di-AMP sodium salt

CAS number: 54447-84-6

Formula: C20H22N10O12P2 •2Na

Molecular weight: 702.38 g/mol

Purity: ≥ 95% by LC/MS

Solubility: 50 mg/ml in water

Quality control:

  • Sterility guaranteed
  • The absence of bacterial contamination (e.g. lipoproteins and endotoxins) has been confirmed using HEK-Blue™ TLR2 and HEK‑Blue™ TLR4 cells
  • Endotoxin level < 5 EU/mg (measurement by kinetic chromogenic LAL assay)
  • The biological activity of  c-di-AMP VacciGrade™ has been assessed by measuring the induction of the interferon pathway in THP1-Blue™ ISG cells
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Contents

  • 1 mg of lyophilized c-di-AMP VacciGrade™
  • 10 ml sterile endotoxin-free physiological water (NaCl 0.9%)

c-di-AMP VacciGrade™ is shipped at room temperature

c-di-AMP VacciGrade™  should be stored at -20°C.

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VacciGrade™

VacciGrade™ is a high-quality pre-clinical grade. VacciGrade™ products are filter-sterilized (0.2 µm) and filled under strict aseptic conditions in a clean room*. The absence of bacterial contamination is assessed by a sterility test using a pharmacopeia-derived assay. The level of bacterial contaminants (endotoxins and lipoproteins) in each lot is verified using a LAL assay and/or a TLR2 and TLR4 reporter assay.
*Except for LPS VacciGrade™, which is prepared in a laminar flow hood dedicated to LPS.

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