CFA Unit size Cat. code Docs Qty Price
Complete Freund’s Adjuvant
10 ml
6 x 10 ml

Vaccine Adjuvant: Complete Freund’s Adjuvant

Complete Freund's Adjuvant (CFA) consists of heat-killed Mycobacterium tuberculosis in non-metabolizable oils (paraffin oil and mannide monooleate). CFA contains trehalose 6,6’ dimycolate (TDM), which stimulates Mincle. Additionally, CFA has ligands for TLR2, TLR4, and TLR9.

CFA can be used to produce water-in-oil emulsions of antigens.

Injection of antigen in CFA induces a Th1-dominated response when compared to injection in Incomplete Freund's Adjuvant (IFA), which lacks mycobacterial components and induces a Th2-dominated response.

An essential component of CFA-mediated response is an intense inflammatory reaction at the site of antigen deposition. For most applications, CFA is usually only necessary for the initial immunization, while IFA is the adjuvant of choice for subsequent immunizations.


CFA is for research use only, not for use in humans.

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Specificity: Th1 response

VacciGrade™ (preclinical grade).

Quality: Sterile, Endotoxin level < 1 EU/ml

Quality control

  • CFA is VacciGrade™ (preclinical grade). It is prepared under strict aseptic conditions and is tested for the presence of endotoxins. CFA is guaranteed sterile and its endotoxin level is < 1 EU/ml.
  • Adjuvanticity of CFA was evaluated by assessing the levels of total mouse IgGs (mIgGs) and the mIgG1 and mIgG2 isotypes after an initial subcutaneous injection of EndoFit™ Ovalbumin/CFA (1:1, v/v) in mice. To minimize side-effects, EndoFit™ Ovalbumin/IFA (1:1, v/v) was used for a subcutaneous boost injection. Results were compared to mice which received the antigen alone.
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CFA is available in two quantities:

  • 10 ml: cat. code # vac-cfa-10
  • 6 x 10 ml: cat. code # vac-cfa-60

Each 10 ml CFA vial contains 10 mg heat killed dried Mycobacterium tuberculosis (strain H37Ra), 1.5 ml Mannide Monooleate and 8.5 ml Paraffin oil.

CFA is shipped at room temperature.

CFA should be stored at 4˚C.

CFA is stable for 2 years.

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