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Incomplete Freund's Adjuvant
6 x 10 ml
Vaccine Adjuvant: Incomplete Freund′s adjuvant
IFA (Incomplete Freund’s adjuvant) is one of the most commonly used adjuvants in research. It is prepared from non-metabolizable oils.
- IFA can be used to produce water-in-oil emulsions of antigens.
- IFA induces a predominantly Th2-biased response through the formation of a depot at the injection site and the stimulation of antibody producing plasma cells.
IFA is VacciGrade™ (preclinical grade) for research use only, not for use in humans.
Specificity: Th2 response
Working concentration: 1:1 (IFA:antigen)
Quality: Sterile, Endotoxin level < 1 EU/ml.
Appearance: Clear, pale yellow liquid
- IFA is VacciGrade™ (preclinical grade). It is prepared under strict aseptic conditions and is tested for the presence of endotoxins. IFA is guaranteed sterile and its endotoxin level is < 1 EU/ml.
- Adjuvanticity of IFA was evaluated by assessing the levels of total mouse IgGs (mIgGs) and the mIgG1 and mIgG2 isotypes after two consecutive subcutaneous injections of EndoFit™ Ovalbumin/IFA (1:1, v/v) in mice. Results were compared to mice which received the antigen alone.
IFA (Incomplete Freund’s Adjuvant) is provided as a sterile ready-to-use liquid.
IFA is available in two quantities:
- 10 ml: cat. code # vac-ifa-10
- 6 x 10 ml: cat. code # vac-ifa-60
Each 10 ml IFA vial contains 1.5 ml Mannide Monooleate and 8.5 ml Paraffin oil.
IFA is shipped at room temperature
IFA should be stored at 4°C.
IFA is stable for 6 months when properly stored.
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IFA (Incomplete Freund’s adjuvant) is one of the most commonly used adjuvants in research.
It is prepared from non-metabolizable oils (paraffin oil and mannide monooleate) . IFA does not contain killed Mycobacterium tuberculosis found in Complete Freund's Adjuvant (CFA) and is thus less inflammatory . IFA induces a predominantly Th2-biased response through the formation of a depot at the injection site and the stimulation of antibody producing plasma cells . It has been suggested that NOD2 modulates the adjuvant effects of IFA . IFA is utilized to produce water-in-oil emulsions of antigens. It is routinely used for boosting immunizations subsequent to CFA. It can also be used for the initial immunization, particularly when a strong antigen is chosen or moderate antibody levels are sufficient.
1. Lindblad EB., 2000. Freund’s Adjuvants. In: Vaccine adjuvants: Preparation Methods and Research Protocols. Humana Press.Totowa, NJ.
2. Petrovsky N. & Aguilar JC., 2004. Vaccine adjuvants: Current state and future trends. Immunol Cell Biol. 82(5): 488-96.
3. Moreira L0. et al., 2008. Modulation of adaptive immunity by different adjuvant-antigen combinations in mice lacking Nod2. Vaccine 26(46): 5808-13.