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IFA

IFA Unit size Cat. code Docs Qty Price
Incomplete Freund's Adjuvant
10 ml
6 x 10 ml
vac-ifa-10
+-
$56.00

Vaccine Adjuvant: Incomplete Freund′s adjuvant

IFA (Incomplete Freund’s adjuvant) is one of the most commonly used adjuvants in research. It is prepared from non-metabolizable oils.

  • IFA can be used to produce water-in-oil emulsions of antigens.
  • IFA induces a predominantly Th2-biased response through the formation of a depot at the injection site and the stimulation of antibody producing plasma cells.

 

IFA is VacciGrade™ (preclinical grade) for research use only, not for use in humans.

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Specifications

Specificity: Th2 response

Working concentration: 1:1 (IFA:antigen)

Quality: Sterile, Endotoxin level < 1 EU/ml.

Appearance: Clear, pale yellow liquid

Quality control

  • IFA is VacciGrade™ (preclinical grade). It is prepared under strict aseptic conditions and is tested for the presence of endotoxins. IFA is guaranteed sterile and its endotoxin level is < 1 EU/ml.
  • Adjuvanticity of IFA was evaluated by assessing the levels of total mouse IgGs (mIgGs) and the mIgG1 and mIgG2 isotypes after two consecutive subcutaneous injections of EndoFit™ Ovalbumin/IFA (1:1, v/v) in mice. Results were compared to mice which received the antigen alone.
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Contents

IFA (Incomplete Freund’s Adjuvant) is provided as a sterile ready-to-use liquid.

IFA is available in two quantities:

  • 10 ml: cat. code # vac-ifa-10
  • 6 x 10 ml: cat. code # vac-ifa-60

Each 10 ml IFA vial contains 1.5 ml Mannide Monooleate and 8.5 ml Paraffin oil.

 

IFA is shipped at room temperature

IFA should be stored at 4°C.

IFA is stable for 6 months when properly stored.

DO NOT FREEZE.

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Description

IFA (Incomplete Freund’s adjuvant) is one of the most commonly used adjuvants in research.

It is prepared from non-metabolizable oils (paraffin oil and mannide monooleate) [1]. IFA does not contain killed Mycobacterium tuberculosis found in Complete Freund's Adjuvant (CFA) and is thus less inflammatory [1]. IFA induces a predominantly Th2-biased response through the formation of a depot at the injection site and the stimulation of antibody producing plasma cells [2]. It has been suggested that NOD2 modulates the adjuvant effects of IFA [3]. IFA is utilized to produce water-in-oil emulsions of antigens. It is routinely used for boosting immunizations subsequent to CFA. It can also be used for the initial immunization, particularly when a strong antigen is chosen or moderate antibody levels are sufficient.

 

1. Lindblad EB., 2000. Freund’s Adjuvants. In: Vaccine adjuvants: Preparation Methods and Research Protocols. Humana Press.Totowa, NJ.
2. Petrovsky N. & Aguilar JC., 2004. Vaccine adjuvants: Current state and future trends. Immunol Cell Biol. 82(5): 488-96.
3. Moreira L0. et al., 2008. Modulation of adaptive immunity by different adjuvant-antigen combinations in mice lacking Nod2. Vaccine 26(46): 5808-13.

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Citations

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