Invivogen
Menu

IFA

IFA Unit size Cat. code Docs Qty Price
Incomplete Freund's Adjuvant
10 ml
6 x 10 ml
vac-ifa-10
+-
$64.00

Vaccine Adjuvant: Incomplete Freund′s adjuvant

IFA (Incomplete Freund’s adjuvant) is one of the most commonly used adjuvants in research. It is prepared from non-metabolizable oils.

  • IFA can be used to produce water-in-oil emulsions of antigens.
  • IFA induces a predominantly Th2-biased response through the formation of a depot at the injection site and the stimulation of antibody-producing plasma cells.

 

IFA is VacciGrade™, a high-quality pre-clinical grade.

IFA is for research use only, and not for human or veterinary use.

Back to the top

Specifications

Specificity: Th2 response

VacciGrade™ (preclinical grade)

Working concentration: 1:1 (IFA:antigen)

Appearance: Clear, pale yellow liquid

Quality control

  • Sterility guaranteed
  • Endotoxin level < 1 EU/ml (measurement by kinetic chromogenic LAL assay)
  • Adjuvanticity of IFA was evaluated by assessing the levels of total mouse IgGs (mIgGs) and the mIgG1 and mIgG2 isotypes after two consecutive subcutaneous injections of EndoFit™ Ovalbumin/IFA (1:1, v/v) in mice. Results were compared to mice that received the antigen alone.
Back to the top

Contents

IFA (Incomplete Freund’s Adjuvant) is provided as a sterile ready-to-use liquid.

IFA is available in two quantities:

  • 10 ml: cat. code # vac-ifa-10
  • 6 x 10 ml: cat. code # vac-ifa-60

Each 10 ml IFA vial contains 1.5 ml Mannide Monooleate and 8.5 ml Paraffin oil.

 

IFA is shipped at room temperature

IFA should be stored at 4°C.

IFA is stable for 2 years when properly stored.

DO NOT FREEZE.

Back to the top

Details

IFA (Incomplete Freund’s adjuvant) is one of the most commonly used adjuvants in research.

It is prepared from non-metabolizable oils (paraffin oil and mannide monooleate) [1]. IFA does not contain killed Mycobacterium tuberculosis found in Complete Freund's Adjuvant (CFA) and is thus less inflammatory [1]. IFA induces a predominantly Th2-biased response through the formation of a depot at the injection site and the stimulation of antibody producing plasma cells [2]. It has been suggested that NOD2 modulates the adjuvant effects of IFA [3]. IFA is utilized to produce water-in-oil emulsions of antigens. It is routinely used for boosting immunizations subsequent to CFA. It can also be used for the initial immunization, particularly when a strong antigen is chosen or moderate antibody levels are sufficient.

 

1. Lindblad EB., 2000. Freund’s Adjuvants. In: Vaccine adjuvants: Preparation Methods and Research Protocols. Humana Press.Totowa, NJ.
2. Petrovsky N. & Aguilar JC., 2004. Vaccine adjuvants: Current state and future trends. Immunol Cell Biol. 82(5): 488-96.
3. Moreira L0. et al., 2008. Modulation of adaptive immunity by different adjuvant-antigen combinations in mice lacking Nod2. Vaccine 26(46): 5808-13.

Back to the top

VacciGrade™

VacciGrade™ is a high-quality pre-clinical grade. VacciGrade™ products are filter-sterilized (0.2 µm) and filled under strict aseptic conditions in a clean room*. The absence of bacterial contamination is assessed by a sterility test using a pharmacopeia-derived assay. The level of bacterial contaminants (endotoxins and lipoproteins) in each lot is verified using a LAL assay and/or a TLR2 and TLR4 reporter assay.
*Except for LPS VacciGrade™, which is prepared in a laminar flow hood dedicated to LPS.

Back to the top
Customer Service
& Technical Support
Shopping cart is empty