pSELECT-Tag is a family of expression plasmids designed to generate tagged proteins in order to facilitate their detection and/or purification.
pSELECT-Tag features three well-known tags: the green fluorescent protein (GFP) gene, the human influenza hemagglutinin (HA) epitope, the polyhistidine (His) tag, and , a novel secreted luciferase (Lucia).
pSELECT-Tag plasmids allow the addition of the tag at either the N or C terminus of the protein of interest.
Applications
- GFP-Tag: Visualization of the spatial and temporal localization of the tagged protein by fluorescence microscopy
- HA-Tag: Detection of the tagged protein by immunocytochemistry, immunoprecipitation or Western blotting, or purification of the tagged protein by affinity chromatography
- His-Tag: Purification of the tagged protein using an NI-NTA column
- Lucia-Tag: Detection and quantification of the tagged protein by bioluminescence assay