pSELECT His-Tag

Expression plasmid with His Tag

ABOUT

pSELECT His-Tag is a family of expression plasmids designed to generate tagged proteins in mammalian cells with the polyhistidine (His) tag in order to purify the tagged protein using an NI-NTA column. pSELECT His-Tag plasmids allow to add the tag either at the N or C terminus of the protein of interest. pSELECT His-Tag plasmids are available with Blasticidin or Zeocin™ resistance gene for selection.

N-Tagged or C-Tagged Protein
- N-terminal tag: the tag encompasses the Start Codon and is followed by a multiple cloning site (MCS)
- C-terminal tag: the tag is cloned downstream of a multiple cloning site and followed by a Stop codon

All products are for research use only, and not for human or veterinary use.

SPECIFICATIONS

Specifications

Tag
His, N-ter
Plasmid backbone
pSELECT
Antibiotic resistance
Zeocin®
Gene promoter
Human EF1α/HTLV hybrid promoter
Subclone start
AgeI
Reconstitution buffer
Endotoxin-free water (not provided)
Tested applications

In vitro transfection

Quality control

Plasmid construct has been confirmed by restriction analysis and sequencing.

CONTENTS

Contents

  • Product: 
    pSELECT-NHis-zeo
  • Cat code: 
    psetz-nhis
  • Quantity: 
    20 µg
Includes:

1 ml of Zeocin™  at 100 mg/ml

Shipping & Storage

  • Shipping method:  Room temperature

    • Storage:

    • -20°C

    Caution:

    • Avoid repeated freeze-thaw cycles

DOCUMENTS

Documents

pSELECT-NHis-zeo

Technical Data Sheet

Plasmid Sequence

Safety Data Sheet

Certificate of analysis

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CUSTOMER SERVICE & TECHNICAL SUPPORT

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