IAP inhibitor - BV6

Product Unit size Cat. code Docs. Qty. Price


Inhibitor of IAPs - Smac mimetic - InvitroFit™

Show product

5 mg


Antagonist of IAPs

Activation of RIPK1-mediated cell death using BV6
Activation of RIPK1-mediated cell death using BV6

BV6 is a potent and specific antagonist of at least three inhibitors of apoptosis proteins (IAPs), namely cIAP1, cIAP2, and XIAP [1]. IAPs exert their anti-apoptotic actions through ubiquitylation of RIPK1 (receptor-interacting serine/threonine-protein kinase 1 ), which results in RIPK1 proteasomal degradation [2]. RIPK1 can no longer recruit RIPK3 and MLKL to form the necrosome and promote necroptosis upon engagement of death receptors.

Learn more Learn more on apoptosis and necroptosis


Mode of action:

BV6 acts as a Smac mimetic. Smac/DIABLO (second mitochondria-derived activator of caspases/direct IAP-binding protein with low isoelectric point) is a natural antagonist of IAPs, which is released in the cytosol in response to pro-apoptotic stimuli.  Upon binding to cIAP, BV6 induces its ubiquitination and rapid proteasomal degradation, enabling the deubiquitination of RIPK1. Thus, BV6 acts as an inducer of apoptosis and necroptosis initiated by death signals such as TNF-α [1, 3].

Note: For full induction of necroptosis upon death receptor engagement, blockade of CASP-8 is also required [4]. This can be achieved by using the CASP-8 inhibitor, Z-IETD-FMK.


Key features:

  • Potent and selective inhibitor of cIAP1, cIAP2, and XIAP
  • Allows RIPK1-mediated apoptosis or necroptosis
  • InvitroFit™ grade: each lot is highly pure (≥95%) and functionally tested



1. Varfolomeev E. et al., 2007. IAP antagonists induce autoubiquitination of c-IAPs, NF-kB Activation, and TNFα-dependent apoptosis. Cell. 131(4):669-681.
2. Annibaldi A. et al., 2018. Ubiquitin-mediated regulation of RIPK1 kinase activity independent of IKK and MK2. Molecular Cell. 69:566-580.
3. Li W. et al., 2011. BV6, an IAP antagonist, activates apoptosis and enhances radiosensitization of non-small cell lung carcinoma in vitro. J. Thorac. Oncol. 6(11), 1801-1809.
4. Choi M.E. et al., 2019. Necroptosis: a crucial pathogenic mediator of human disease. JCI Insight. 4(15):e128834.


Dose-dependent induction of necroptosis
Dose-dependent induction of necroptosis

BV6 contributes to TNF-α-induced cell death in a dose-dependent manner. THP1-HMGB1-Lucia™ cells were incubated with recombinant human TNF-α (100 ng/ml), Z-VAD-FMK (pan-caspase inhibitor, 25 µM), and increasing concentrations of BV6 (cIAP inhibitor). After overnight incubation, the level of HMGB1::Lucia released in the supernatant was assessed by measuring the light signal produced using the QUANTI-Luc™ detection reagent. Data are shown as a percentage (%) of maximal cell death induction.

Back to the top


CAS number: 1001600-56-1

Synonyms: BV-6; N,N’-(hexane-1,6-diyl)bis(1-{(2S)-2-cyclohexyl-2-[(N-methyl-L-alanyl)amino]acetyl}-L-prolyl-beta-phenyl-L-phenylalaninamide)

Working concentration: 1-20 µM for cell culture assays

Solubility: 6 mg/ml (5 mM) in DMSO

Formula: C70H96N10O8

Molecular weight: 1205.6 g/mol

Quality control:

  • Purity: ≥95% (UHPLC)
  • The inhibitory activity of the product has been validated using in-house cellular assays with THP1-HMGB1-Lucia™ cells.
  • The absence of bacterial contamination (e.g. lipoproteins and endotoxins) has been confirmed using HEK-Blue™ TLR2 and HEK-Blue™ TLR4 cells.
Back to the top


  • 5 mg BV6 (provided as a powder)

 BV6 is shipped at room temperature.

 Upon receipt, store at -20 °C.

 Resuspended product is stable for at least 6 months when properly stored.

Alert Avoid repeated freeze-thaw cycles.

Back to the top


Chemical structure of BV6:

Chemical structure of BV6

Back to the top
Customer Service
& Technical Support
Shopping cart is empty