Recombinant human TNF-α
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Cat.code:
rcyc-htnfa
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ABOUT
Human TNF-α protein - Mammalian cell-expressed, tag-free, with HSA
Recombinant human TNF-α is a high-quality and biologically active cytokine, validated using proprietary TNF-α reporter cells. This pro-inflammatory cytokine of the TNF superfamily is produced in CHO cells to ensure protein glycosylation and bona fide 3D structure.
Recombinant human TNF-α can be used together with HEK-Blue™ TNF-α cells for the screening of inhibitory molecules, such as Adalimumab, a therapeutic monoclonal antibody that targets TNF-α and prevents its binding to its receptor (see figures).
Key features
- Each lot is validated using HEK-Blue™ TNF-α cells
- Endotoxin ≤0.1 EU/µg
- 0.2 µm sterile-filtered
Applications
- TNF-α detection and quantification assays (positive control)
- Screening and release assays for antibodies blocking TNF-α signaling
- Screening and release assays for engineered TNF-α proteins
Tumor necrosis factor-alpha (TNF-α) is a pleiotropic cytokine that plays a central role in inflammation, cell apoptosis, and immune system development. This potent mediator of inflammation is implicated in the pathogenesis of several autoimmune and inflammatory diseases such as rheumatoid arthritis and inflammatory bowel disease.
All InvivoGen products are for internal research use only, and not for human or veterinary use.
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SPECIFICATIONS
Specifications
P01375
100 μg/ml in water
Phosphate buffer saline (pH 7.4), 5% saccharose, 0.1% HSA
0.2 µm filtration
The absence of bacterial contamination (e.g. lipoproteins and endotoxins) is confirmed using HEK-Blue™ TLR2 and HEK-Blue™ TLR4 cells.
Cellular assays (tested), ELISA
Each lot is functionally tested and validated.
CONTENTS
Contents
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Product:Recombinant human TNF-α
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Cat code:rcyc-htnfa
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Quantity:20 µg
1.5 ml endotoxin-free water
Shipping & Storage
- Shipping method: Room temperature
- -20°C
- Avoid repeated freeze-thaw cycles
Storage:
Caution:
Details
Tumor necrosis factor-alpha (TNF-α) is a pleiotropic cytokine involved in necrotic and apoptotic cell death, cellular differentiation, inflammation, and regulation of immune cell activity [1]. Notably, deregulated TNF-α production has been implicated in a variety of conditions, including autoimmune and inflammatory diseases [1].
TNF-α is mainly produced by activated monocytes, macrophages, and T cells. It is first synthesized as a membrane-bound molecule that forms a compact homotrimer through non-covalent interactions. The trimeric membrane-bound form is cleaved by tumor necrosis factor-alpha converting enzyme (TACE) releasing the soluble trimer [2]. Both the membrane-bound and soluble TNF-α bind homotrimeric transmembrane receptors, TNFR1 or TNFR2, triggering signaling pathways that involve TRADD, TRAF2, and RIP, and leading to the activation of NF-κB and MAPK pathways.
1. Steeland S. et al., 2018. A new venue of TNF targeting. Int. J. Mol. Sci. 19:1442.
2. Brenner D. et al., 2015. Regulation of tumour necrosis factor signalling: live or let die. Nat Rev Immunol. 15(6):362-74.
DOCUMENTS
Documents
Technical Data Sheet
Validation Data Sheet
Safety Data Sheet
Certificate of analysis
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