Recombinant human TGF-β1
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Recombinant Cytokine, source: HEK293
Recombinant human transforming growth factor-beta (with HSA)
Transforming growth factor-beta (TGF-β), also known as tumor growth factor-beta, is a secreted cytokine that regulates numerous cellular functions, such as cell proliferation, apoptosis, differentiation and migration[1, 2].
TGF-β exists in at least three isoforms; TGF-β1, TGF-β2 and TGF-β3. In the immune system, TGF-β1 is the predominant isoform .
It is produced by many cell types, including macrophages, in a latent form which is bound to two other polypeptides, latent TGF-β1 binding protein (LTBP) and latency-associated peptide (LAP).
Upon cleavage of these proproteins, the mature TGF-β1 is released. This mature protein can bind its cell surface receptors and initiate signaling.
Recombinant human TGF-β1 is intended for use in cell culture applications. It is produced in mammalian cells.
1 - Travis MA. & Sheppard D., 2014. TGF-β activation and function in immunity. Annu Rev Immunol. 32:51-82.
2 - Taylor AW., 2009. Review of the activation of TGF-beta in immunity. J Leukoc Biol. 85(1):29-33.
Source: Mammalian; HEK293 cells
UniProt ID: P01137
Formulation: Recombinant human TGF-β1 was lyophilized from a 0.2 mm filtered phosphate buffer solution (pH 7.4) containing human serum albumin (HSA).
Purity: >95%, as determined by SDS-PAGE.
Molecular mass: ~ 24 kDa in non-reducing conditions
Solubility: 100 μg/ml in water
Endotoxin level: < 0.1 EU/µg as determined by LAL assay
Bioactivity: Validated using HEK-Blue™ TGF-β cellsBack to the top
- 10 μg of TGFβ1 provided as a white lyophilized powder
- 1.5 ml endotoxin-free water
TGFβ1 is shipped at room temperature.
Upon receipt TGFβ1 should be stored at -20°C.Back to the top