Recombinant human CD40L protein - Bioactive cytokine
| Product | Unit size | Cat. code | Docs. | Qty. | Price | |
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Recombinant human CD40L Recombinant Cytokine, source: E. coli |
Show product |
10 µg 5 x 10µg |
rcyec-hcd40l
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Human CD40L protein - Mammalian cell-expressed, tag-free, with HSA
Recombinant human CD40L is a high-quality and biologically active cytokine, validated using proprietary CD40L reporter cells. This member of the TNF superfamily is produced in E. coli and thoroughly purified to remove endotoxins.
Recombinant human CD40L can be used together with HEK-Blue™ CD40L cells for the screening of inhibitory molecules, such as Frexalimab, a monoclonal antibody targeting CD40L (see figures).
CD40L signaling and biological functions
InvivoGen also offers:
Key features
- Each lot is validated using HEK-Blue™ CD40L cells
- Endotoxin ≤ 1 EU/µg
- 0.2 µm sterile-filtered
Applications
- Standard for CD40L detection and quantification assays
- Screening and release assays for antibodies blocking CD40L signaling
- Screening and release assays for engineered CD40L
CD40 Ligand (CD40L) is a protein that is primarily expressed on activated T cells and is a member of the tumor necrosis factor superfamily. Of note, CD40L, together with its receptor CD40, plays a pivotal role in cellular and humoral immunity.
InvivoGen’s products are for research use only, and not for clinical or veterinary use.
Figures
Specifications
Source: E. coli
Species: Human
Alternative names: CD154, T-cell antigen Gp39, TNF-related activation protein (TRAP), Tumor necrosis factor ligand superfamily member 5
Carrier: HSA (human serum albumin)
Tag: Tag-free
Accession number: P29965
Molecular weight: ~ 16 kDa (SDS-PAGE)
Solubility: 100 μg/ml in water
Formulation: Phosphate buffer saline (pH 7.4), 5% saccharose, 2% HSA
Sterility: 0.2 µm filtration
Form: Lyophilized
Reconstitution buffer: Endotoxin-free water (provided)
Purity: ≥95% (SDS-PAGE)
Endotoxin: The absence of bacterial contamination (e.g. lipoproteins and endotoxins) has been confirmed using HEK-Blue™ TLR2 and HEK‑Blue™ TLR4 cells.
Tested applications: Cellular assays
Quality control: Each lot is functionally tested and validated.
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Recombinant human CD40L is provided as a lyophilized powder and is available in two quantities:
- rcyec-hcd40l: 10 μg
- rcyec-hcd40l-5: 50 μg (5 x 10 μg)
- 1.5 ml endotoxin-free water for rcyec-hcd40l and rcyec-hcd40l-5
Recombinant human CD40L is shipped at room temperature.
Upon receipt, the product should be stored at -20 °C.
Avoid repeated freeze-thaw cycles.
Details
CD40 Ligand (CD40L), also known as CD154, TRAP, or gp39, is a type II transmembrane glycoprotein belonging to the tumor necrosis factor (TNF) family. It is mainly expressed in CD4+-T cells and interacts with CD40 on antigen-presenting cells to regulate both humoral and cellular immune responses [1-3].
The CD40 cytoplasmic domain binds directly to several TNF receptor-associated factors (TRAFs), and this interaction is thought to initiate CD40 signaling. CD40-mediated signaling results in NF-κB, c-Jun N-terminal kinase (JNK), and p38 mitogen-activated protein kinase (MAPK) activation. CD40L-CD40 interactions are thought to play an important role in the pathogenesis of many diseases [4, 5].
1. Karnell J.L. et al., 2019. Targeting the CD40-CD40L pathway in autoimmune diseases: Humoral immunity and beyond. Adv Drug Deliv Rev. 141:92-103.
2. Laman J.D. et al., 2017. Functions of CD40 and Its Ligand, gp39 (CD40L). Crit Rev Immunol. 37(2-6):371-420.
3. Elgueta R. et al., 2009. Molecular mechanism and function of CD40/CD40L engagement in the immune system. Immunol. Rev. 229, 152–172.
4. Seijkens T. et al., 2013. CD40–CD40L: Linking pancreatic, adipose tissue and vascular inflammation in type 2 diabetes and its complications. Diabetes and Vascular Disease Research. 10: 115-122.
5. Daoussis D. et al., 2004. Targeting CD40L: a promising therapeutic approach. Clin Diagn Lab Immunol. 11(4):635-41.