Anti-hCD40L-lgG

Neutralizing monoclonal antibody against human CD40L

Neutralizing monoclonal antibody against human CD40L

Anti-hCD40L-IgG is a monoclonal mouse IgG2b antibody specific for human CD40 ligand (hCD40L). This antibody was generated by InvivoGen using DNA immunization. It has been produced in hybridomas and purified by affinity chromatography.

This antibody has been selected for its ability to efficiently neutralize the biological activity of hCD40L. CD40 Ligand (CD40L) is a protein that is primarily expressed on activated T cells and is a member of the tumor necrosis factor superfamily. Of note, CD40L, together with its receptor CD40, plays a pivotal role in cellular and humoral immunity [1-3].

More details

Key features:

• Reacts with human CD40L
• Provided azide-free
• Each lot is functionally tested

References:

1. Karnell J.L. et al., 2019. Targeting the CD40-CD40L pathway in autoimmune diseases: Humoral immunity and beyond. Adv Drug Deliv Rev. 141:92-103.
2. Laman J.D. et al., 2017. Functions of CD40 and Its Ligand, gp39 (CD40L). Crit Rev Immunol. 37(2-6):371-420.
3. Elgueta R. et al., 2009. Molecular mechanism and function of CD40/CD40L engagement in the immune system. Immunol. Rev. 229, 152–172.

Figures

Anti-hCD40L-IgG mAb is a potent neutralization antibody. Increasing concentrations of Anti-hCD40L-hIgG monoclonal antibody (mAb) were incubated with 100 ng/ml of recombinant human CD40L for 30 minutes prior to the addition of the HEK-Blue™ CD40L cells. After overnight incubation, the SEAP response was determined using QUANTI-Blue™, a SEAP detection reagent. Data are presented as percentage of neutralization (mean ± SEM).

Specifications

Target: Natural and recombinant human CD40 Ligand (CD40L)

Clone: 13H5

Isotype: Mouse IgG2b, kappa

Control: Mouse IgG2b Control

Source: Hybridoma cells

Immunogen: Human CD40L protein expressed in Swiss mice following DNA immunization

Formulation: 0.2 µm filtered solution in a sodium phosphate buffer with glycine, saccharose, and stabilizing agents

Tested applications: Neutralizing antibody. It can also be used for detection using flow cytometry.

Quality control:

• This product has been validated for neutralization using cellular assays.
• The absence of bacterial contamination (e.g. lipoproteins and endotoxins) has been confirmed using HEK-Blue™ TLR2 and HEK‑Blue™ TLR4 cells.

Contents

3 x 100 µg of purified Anti-hCD40L-IgG antibody provided azide-free and lyophilized

Product is shipped at room temperature.

Upon receipt, store lyophilized antibody at -20 °C.

Details

CD40 Ligand (CD40L), also known as CD154, TRAP, or gp39, is a type II transmembrane glycoprotein belonging to the tumor necrosis factor (TNF) family. It is mainly expressed in CD4+-T cells and interacts with CD40 on antigen-presenting cells to regulate both humoral and cellular immune responses [1-3].

The CD40 cytoplasmic domain binds directly to several TNF receptor-associated factors (TRAFs), and this interaction is thought to initiate CD40 signaling. CD40-mediated signaling results in NF-κB, c-Jun N-terminal kinase (JNK), and p38 mitogen-activated protein kinase (MAPK) activation. CD40L-CD40 interactions are thought to play an important role in the pathogenesis of many diseases [4, 5].

1. Karnell J.L. et al., 2019. Targeting the CD40-CD40L pathway in autoimmune diseases: Humoral immunity and beyond. Adv Drug Deliv Rev. 141:92-103.
2. Laman J.D. et al., 2017. Functions of CD40 and Its Ligand, gp39 (CD40L). Crit Rev Immunol. 37(2-6):371-420.
3. Elgueta R. et al., 2009. Molecular mechanism and function of CD40/CD40L engagement in the immune system. Immunol. Rev. 229, 152–172.
4. Seijkens T. et al., 2013. CD40–CD40L: Linking pancreatic, adipose tissue and vascular inflammation in type 2 diabetes and its complications. Diabetes and Vascular Disease Research. 10: 115 - 122.
5. Daoussis D. et al., 2004. Targeting CD40L: a promising therapeutic approach. Clin Diagn Lab Immunol. 11(4):635-41.