Anti-hSTING-IgG
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Cat.code:
mabg-hstg-2
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ABOUT
Anti-human STING monoclonal antibody for detection
Anti-hSTING-IgG (clone 3B5) is a monoclonal mouse IgG1 antibody against human STING (hSTING). This antibody was generated by InvivoGen using DNA immunization. It has been produced in hybridomas and purified by affinity chromatography.
Anti-hSTING-IgG has been selected for its ability to bind hSTING. It recognizes the most prevalent isoform, “wild‑type” R232 and HAQ hSTING variants. This antibody can be used to detect hSTING using ELISA and Western blot.
STING (stimulator of interferon genes) is essential for the interferon response to cytoplasmic foreign or self-DNA. It directly senses cyclic dinucleotides (CDNs), which are important messengers in bacteria and innate immune agonists in mammals [1]. Several STING variants, which differ in their responses to CDNs, have been described in the human population [2].
Key features
- Reacts with the “wild‑type” R232 (R71‑G230-R232-R293) hSTING variant
- Reacts with the HAQ (H71‑A230-R232-Q293) hSTING variant
- Provided azide-free
- Each lot is functionally tested
Applications
- Western blot
- ELISA
References:
1. Sun L. et al., 2013. Cyclic GMP-AMP synthase is a cytosolic DNA sensor that activates the type I interferon pathway. Science. 339:786-91.
2. Yi G. et al., 2013. Single nucleotide polymorphisms of human STING can affect innate immune response to cyclic dinucleotides. PLOS One. 8:e77846.
All products are for research use only, and not for human or veterinary use.
SPECIFICATIONS
Specifications
STING
Human
Western blot (tested), ELISA (tested)
Sodium phosphate buffer, glycine, saccharose, stabilizing agents
Negative (tested using EndotoxDetect™ assay)
Each lot is functionally tested and validated.
CONTENTS
Contents
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Product:Anti-hSTING-IgG
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Cat code:mabg-hstg-2
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Quantity:2 x 100 µg
Shipping & Storage
- Shipping method: Room temperature
- -20°C
- Avoid repeated freeze-thaw cycles
Storage:
Caution:
Details
STING (stimulator of interferon genes) has become a focal point in immunology research and drug discovery [1, 2]. In a healthy individual, STING functions as a signaling hub, orchestrating immune responses to pathogenic, tumoral, or self-DNA detected in the cytoplasm [2]. Upon activation, STING induces type I interferon (IFN) production through TANK-binding-kinase-I (TBK1)-mediated IFN regulatory factor (IRF3) signaling [2]. STING activation also leads to NF‑κB-dependent inflammatory cytokine production [2]. In some autoimmune diseases such as STING-associated vasculopathy with onset in infancy (SAVI), STING is constitutively activated resulting in high IFN production [3, 4]. The discovery of a mechanism to pharmacologically inhibit STING should lead to new treatments for such diseases.
Reference:
1. Haag S.M. et al., 2018. Targeting STING with covalent small-molecule inhibitors. Nature 559:269-73.
2. Ishikawa H. & Barber G.N. 2008. STING is an endoplasmic reticulum adaptor that facilitates innate immune signalling. Nature 455:674-8.
3. Liu Y. et al., 2014. Activated STING in a vascular and pulmonary syndrome. N Engl J Med. 371:507-18.
4. Jeremiah N. et al., 2013. Inherited STING-activating mutation underlies a familial inflammatory syndrome with lupus-like manifestations. J Clin Invest. 124:5516-20.
DOCUMENTS
Documents
Technical Data Sheet
Validation Data Sheet
Safety Data Sheet
Certificate of analysis
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