Anti-hIFN-α-IgG

Neutralizing IgG monoclonal antibody to human interferon-alpha 2

Neutralizing monoclonal antibody against human IFN-α

Anti-hIFN-α-IgG is a monoclonal antibody for human interferon α 2 (hIFN-α2). This antibody was generated by recombinant DNA technology. It has been produced in CHO cells and purified by affinity chromatography.
Anti-hIFN-α-IgG has been selected for its ability to efficiently neutralize the biological activity of hIFN-α2. IFN-α is an important anti-viral cytokine that also has anti-proliferative and immunomodulatory functions [1, 2].

More details

Key features:

• Reacts with hIFN-α1, hIFN-α2, hIFN-α5, hIFN-α8, hIFN-α14, hIFN-α16, hIFN-α17, and hIFN-α21
• Weakly reacts with hIFN-α4 and IFN-α10
• Does not react with hIFN-α6 or hIFN-α7
• Provided azide-free
• Each lot is functionally tested

References:

1. Schreiber G. 2017. The molecular basis for differential type I interferon signaling. J. Biol. Chem. 292:7285-94.
2. McNab F. et al., 2015. Type I interferons in infectious disease. Nat Rev Immunol. 15(2):87-103.

Specifications

Target: Natural and recombinant human interferon-alpha 2 (IFN-α2)

Specificity: Reacts with human IFN-α1, IFN-α2, IFN-α5, IFN-α8, IFN-α14, IFN-α16, IFN-α17 and IFN-α21. Very weakly reacts with human IFN-α4 and IFN-α10. It does not react with IFN-α6 or IFN-α7.

Clone: H7WM116

Isotype: Human IgG1, kappa

Control: Anti-β-Gal-hIgG1

Source: CHO cells

Formulation: 0.2 µm filtered solution in a sodium phosphate buffer with saccharose, glycine, and stabilizing agents

Purity: Purified by affinity chromatography with protein G

Applications: Anti-hIFN-α-IgG is a neutralizing antibody, it blocks hIFN-α-induced cellular activation.

Quality control:

• This product has been validated for neutralization.
• The absence of bacterial contamination (e.g. lipoproteins and endotoxins) has been confirmed using HEK-Blue™ TLR2 and HEK‑Blue™ TLR4 cells.

Contents

• 3 x 100 µg purified anti-hIFN-α-IgG antibody, provided azide-free and lyophilized.

 The product is shipped at room temperature.

 Upon receipt, store lyophilized antibody at -20 °C.

Reconstituted antibody is stable for 1 month at 4 °C and for 1 year at -20 °C.

 Avoid repeated freeze-thaw cycles.

Details

Type I interferons, in particular interferon-alpha (IFN-α) and interferon beta (IFN-β), play a vital role in host resistance to viral infections [1, 2]. The type I IFN family is a multi-gene cytokine family that encodes 13 partially homologous IFN-α subtypes in humans (14 in mice), a single IFN-β, and several poorly defined single gene products (IFN-ɛ, IFN-τ, IFN-κ, IFN-ω, IFN-δ, and IFN-ζ) [1, 2].  IFN-α and IFN-β are the best-defined and most broadly expressed type I IFNs [2].

IFN-β and all of the IFN-α subtypes bind to a heterodimeric transmembrane receptor composed of the subunits IFNAR1 and IFNAR2 which are associated with the tyrosine kinases Tyk2 and Jak1 (Janus kinase 1) respectively. These kinases phosphorylate STAT1 and STAT2 which then dimerize and interact with IFN regulatory factor 9 (IRF9), leading to the formation of the ISGF3 complex. ISGF3 binds to IFN-stimulated response elements (ISRE) in the promoters of IFN-stimulated genes (ISG) to regulate their expression. 

1. Schreiber G. 2017. The molecular basis for differential type I interferon signaling. J. Biol. Chem. 292:7285-94.
2. McNab F. et al., 2015. Type I interferons in infectious disease. Nat Rev Immunol. 15(2):87-103.