Human IgG1 Isotype Control Antibody

E. coli β-Galactosidase antibody (clone T9C6) - Recombinant

ABOUT

Recombinant human IgG1 control mAb against E. coli β-galactosidase

Anti-β-Gal-hIgG1 features the constant region of the human IgG1 isotype and the variable region of Mouse IgG2a Control. Mouse IgG2a Control is a mouse IgG2a monoclonal antibody (mAb) that targets E. coli β-galactosidase (β-Gal), an antigen that is not present in humans or mice. 

This antibody was generated by DNA immunization with a plasmid expressing the β-Gal gene in Swiss mice. It can be used as an isotype control for human IgG1 antibodies.

Human IgG1 is the most abundant immunoglobin present in serum and binds with high affinity to the Fc receptor on phagocytic cells.

The human IgG1 isotype displays high antibody-dependent cell-mediated cytotoxicity (ADCC) and complement-dependent cytotoxicity (CDC).

Anti-β-Gal-hIgG1 was generated by recombinant DNA technology. It has been produced in Chinese hamster ovary (CHO) cells and purified by affinity  chromatography.

All products are for research use only, and not for human or veterinary use.

SPECIFICATIONS

Specifications

Target

β-Galactosidase

Target species

E. coli

Applications

Negative control antibody

Isotype
hIgG1
kappa
Clone
T9C6
Tag
Tag-free
Source
CHO cells
Purification
Protein G
Formulation buffer

Sodium phosphate buffer, glycine, saccharose, stabilizing agents

Preservative
Azide-free
Reconstitution buffer
Sterile water (not provided)
Endotoxin

Negative (tested using EndotoxDetect™ assay)

Tested applications

Negative control for cellular assays, flow cytometry, ELISA

Quality control

Each lot is validated by flow cytometry.

CONTENTS

Contents

  • Product: 
    Anti-β-Gal-hIgG1
  • Cat code: 
    bgal-mab1
  • Quantity: 
    200 µg

Shipping & Storage

  • Shipping method:  Room temperature
  • Storage:

    • -20°C
    Stability: -20°C for up to 1 year

    Caution:

    • Avoid repeated freeze-thaw cycles

DOCUMENTS

Documents

Anti-β-Gal-hIgG1

Technical Data Sheet

Safety Data Sheet

Certificate of analysis

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CUSTOMER SERVICE & TECHNICAL SUPPORT

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