IFN-α/β Reporter HEK 293 Cells
HEK-Blue™ IFN-α/β cells | Unit size | Cat. code | Docs | Qty | Price |
---|---|---|---|---|---|
Human HEK293 cells - Type I IFNs Reporter Cells |
3-7 x 10e6 cells |
hkb-ifnab |
You may also need : QUANTI-Blue™ | View more associated products ▼
Human Type I IFN Reporter Cells
HEK-Blue™ IFN-α/β Cells
HEK-Blue™ IFN-α/β cells allow the detection of bioactive human type I interferons (i.e. IFN-α and IFN-β) by monitoring the activation of the ISGF3 pathway.
These cells were generated by stable transfection of HEK293 cells with the human STAT2 and IRF9 genes to obtain a fully active type I IFN signaling pathway. The other genes of the pathway (IFNAR1, IFNAR2, JAK1, TyK2, and STAT1) are naturally expressed by these cells. The cells feature an inducible SEAP (secreted embryonic alkaline phosphatase) reporter gene under the control of the IFN-α/β inducible ISG54 promoter.
Stimulation of HEK-Blue™ IFN-α/β cells with human IFN-α or IFN-β activates the JAK/STAT/ISGF3 pathway and subsequently induces the production of SEAP. Levels of SEAP are readily assessable in the supernatant using QUANTI-Blue™.
Additionally, the activation of HEK-Blue™ IFN-α/β cells with human IFN-α can be blocked with a neutralizing monoclonal antibody, such as anti-hIFN-α-IgA.
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Antibiotic resistance: blasticidin, Zeocin™
Growth medium: DMEM, 4.5 g/l glucose, 2 mM L-glutamine, 10% (v/v) heat-inactivated fetal bovine serum, 100 U/ml penicillin, 100 µg/ml streptomycin, 100 µg/ml Normocin™
Guaranteed mycoplasma-free
Detects human type I interferons:
- Detection range for human IFN-α: 1 - 104 IU/ml
- Detection range for human IFN-β: 10 - 104 IU/ml
Contents
- 1 vial containing 3-7 x 106 cells
- 1 ml of Blasticidin (10 mg/ml)
- 1 ml of Zeocin™ (100 mg/ml)
- 1 ml Normocin™ (50 mg/ml)
- 1 ml of QB reagent and 1 ml of QB buffer (sufficient to prepare 100 ml of QUANTI-Blue™ Solution, a SEAP detection reagent)
Shipped on dry ice (Europe, USA & Canada)
Details
Type I interferons, in particular interferon alpha (IFN-α) and interferon beta (IFN-β), play a vital role in host resistance to viral infections.
They signal mainly through the JAK-STAT pathway. Following their production, IFN-α and IFN-β bind to a common receptor (IFNAR) and recruit the Janus kinases (JAK1 and TyK2).
JAKs phosphorylate STAT1 and STAT2, which then dimerize and interact with IFN regulatory factor 9 (IRF9), forming a complex named ISGF3.
ISGF3 binds to IFN-stimulated response elements (ISRE) in the promoters of IFN-stimulated genes (ISG) to regulate their expression.
Stimulation of HEK-Blue™ IFN-α/β cells with human IFN-α or IFN-β activates the JAK/STAT/ISGF3 pathway and subsequently induces the production of SEAP.
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