HEK-Blue™ IL-10 Cells
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Cat.code:
hkb-il10v2
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Note: A new clone is provided with an improved IL-10 response. The cat code has been changed accordingly (hkb-il10v2).
ABOUT
IL-10 responsive STAT3-SEAP reporter assay
HEK-Blue™ IL-10 cells are designed to monitor human IL-10-induced STAT3 stimulation or inhibition through SEAP detection. This colorimetric bioassay can be used for screening activatory molecules, such as engineered cytokines, or inhibitory molecules, such as neutralizing antibodies.
HEK-Blue™ IL-10 cells respond strongly to recombinant human IL-10, and to a lower extent to mouse (m)IL-10 (see figures). The reliable and consistent performance of HEK-Blue™ IL-10 cells makes them suitable for release assays of therapeutic molecules that inhibit IL-10 signaling, such as monoclonal antibodies tested using InvivoGen's Anti-hIL-10-hIgG1 mAb.
Key features
- Readily assessable STAT3-inducible SEAP reporter activity
- Convenient readout using QUANTI-Blue™ Solution
- Response to human (h) IL-10 and, to a weaker extent, mouse (m) IL-10
- Stability guaranteed for 20 passages
Applications
- Therapeutic development
- Drug screening
- Release assay
Interleukin 10 (IL-10) is an anti-inflammatory cytokine with a crucial role in preventing inflammatory and autoimmune pathologies. While it is known for its immunosuppressive role in conditions such as inflammatory bowel disease and chronic infections, it also exhibits a surprising protective effect in cancer.
Disclaimer: These cells are for internal research use only and are covered by a Limited Use License (See Terms and Conditions). Additional rights may be available.
SPECIFICATIONS
Specifications
IL-10
Human, Mouse
Detection and quantification of IL-10 activity
Human IL-10: 0.3 - 30 ng/ml
Mouse IL-10: 25 - 1000 ng/ml
Complete DMEM (see TDS)
Verified using Plasmotest™
Each lot is functionally tested and validated.
CONTENTS
Contents
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Product:HEK-Blue™ IL-10 Cells
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Cat code:hkb-il10v2
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Quantity:3-7 x 10^6 cells
- 2 x 1 ml of HEK-Blue™ Selection (250x concentrate)
- 1 ml of Normocin™ (50 mg/ml)
- 1 ml of QB reagent and 1 ml of QB buffer (sufficient to prepare 100 ml of QUANTI-Blue™ Solution, a SEAP detection reagent)
Shipping & Storage
- Shipping method: Dry ice
- Liquid nitrogen vapor
- Upon receipt, store immediately in liquid nitrogen vapor. Do not store cell vials at -80°C.
Storage:
Caution:
Details
Interleukin 10 (IL-10), is the founding member of the IL-10 cytokine family, which comprises IL-10, IL-19, IL-20, IL-22, IL-24, IL-26, IL-28A (IFN-λ2), IL-28B (IFN-λ3), and IL-29 (IFN-λ1)1. IL-10 was originally depicted as a key mediator of anti-inflammatory responses. While the immunosuppressive action of IL-10 is illustrated by its genetic association to inflammatory bowel disease, or its upregulated expression in chronic infections, an unanticipated IL-10 protective role has been reported in cancer [1].
IL-10 also plays important homeostatic roles in non-immune cells, including neurons, adipocytes, and epithelial cells [1]. The manipulation of IL-10 production and signaling has thus become an attractive therapeutic strategy [2].
Upon IL-10 binding, the IL-10Rα chain oligomerizes with the IL-10Rβ chain. This in turn allows the phosphorylation of JAK1 (Janus kinase 1) and TyK2 (Tyrosine kinase 2), and the subsequent activation of STAT3 (Signal transducer and activator of transcription 3) [1]. Importantly, STAT3 is also activated downstream of other cytokine receptors. How the cells trigger distinct and specific gene expression in response to cytokines signaling through the same transcription factor is not fully understood yet [1].
1. Saraiva M, et al., 2019. Biology and therapeutic potential of interleukin-10. J. Exp. Med. doi: 10.1084/jem.20190418.
2. Ouyang W & O’Garra A, 2019. IL-10 family cytokines IL-10 and IL-22: from basic science to clinical translation. Immunity. 50:871-91.
DOCUMENTS
Documents
Technical Data Sheet
Validation Data Sheet
Safety Data Sheet
Certificate of analysis
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