G-CSF Reporter HEK 293 Cells

Cell line description

HEK-Blue™ G-CSF cells were generated from the human embryonic kidney HEK 293 cell line by the stable transfection with the genes encoding for the human CSF receptor (CSFR, aka CD114), human STAT3c, and a STAT3-inducible secreted embryonic alkaline phosphatase (SEAP) reporter. The binding of G-CSF to its receptor triggers a signaling cascade leading to the activation of STAT3 and the subsequent production of SEAP. This can be readily assessed in the supernatant using QUANTI-Blue™ Solution, a SEAP detection reagent.

Granulocyte colony-stimulating factor background

The granulocyte colony-stimulating factor (G-CSF) is a secreted cytokine and hematopoietic growth factor that belongs to the Type I/II cytokine receptor family. G-CSF regulates the differentiation, proliferation, and function of neutrophils [1]. It exerts its biological functions through the formation of a tetramer consisting of two G-CSF molecules and the homodimeric G-CSF receptor (GCSFR, aka CD114). The binding of G-CSF to its receptor triggers activation of the JAK/STAT signaling pathway [1,2]. STAT1, STAT3, and STAT5 are involved in this activation step and the induction of cellular proliferation [1,2]. Studies in humans and mice indicate that while G-CSF contributes to host defense against pathogens, it can also have detrimental effects by promoting inflammatory diseases and cancer [1,2].

​References:

1. Martin KR., et al., 2021. G-CSF — A double-edged sword in neutrophil-mediated immunity. Sem Immunol. 54:101516.
2. Park, DS., et al., 2022. A review of granulocyte colony-stimulating factor receptor signaling and regulation with implications for cancer. Front Oncol. DOI: 10.3389/fonc.2022.932608.