IL-1α & IL-1β Reporter HEK 293 Cells
HEK-Blue™ IL-1R Cells | Unit size | Cat. code | Docs | Qty | Price |
---|---|---|---|---|---|
HEK 293 reporter cells for human and murine IL-1α & IL-1β cytokines |
3-7 x 10e6 cells |
hkb-il1r |
You may also need : QUANTI-Blue™ | View more associated products ▼
Notification: This product is for internal research use only. Additional rights may be available. Please visit InvivoGen’s Terms and Conditions.
Human & Murine IL-1 Reporter Cells
HEK-Blue™ IL-1R Cells signaling pathway
HEK-Blue™ IL-1R cells were designed to detect bioactive human and murine interleukin-1 cytokines (IL‑1α and IL-1β) in various biological samples (cell culture supernatant and serum) by monitoring the activation of the NF-κB and AP-1 pathways. Additionally, these cells detect bioactive IL-1β from cynomolgus monkeys, dogs, and rats. IL‑1 is a secreted pro‑inflammatory cytokine that plays a critical role in immune responses and inflammation [1].
Cell line description:
HEK-Blue™ IL-1R cells endogenously express the human IL-1 receptor and were stably transfected with the murine IL-1 receptor rendering these cells very sensitive to both human and murine IL-1α and IL-1β. HEK-Blue™ IL-1R cells express a SEAP reporter gene under the control of the IFN-β minimal promoter fused to five NF-κB and five AP-1 binding sites. The binding of IL-1β to its receptor IL-1R on the surface of HEK-Blue™ IL-1R cells triggers a signaling cascade leading to the activation NF-κB and the subsequent production of SEAP. Of note, HEK-Blue™ IL-1R derive from HEK-Blue™ IL-1β cells in which the TNF-α response is blocked while the response to murine TNF-α remains intact.
Detection of SEAP in the supernatant of HEK-Blue™ IL-1R cells can be readily assessed using QUANTI-Blue™ Solution, a SEAP detection medium. QUANTI-Blue™ Solution turns blue in the presence of SEAP which can be easily quantified using a spectrophotometer.
Features of HEK-Blue™ IL-1R cells:
- Fully functional IL-1 signaling pathway
- Do not respond to human TNF-α
- Readily assessable SEAP reporter activity
- Functionally tested and guaranteed mycoplasma-free
Applications of HEK-Blue™ IL-1R cells:
- Detection of human and murine IL-1α and IL-1β
- Detection of IL-1 in various biological samples including serum
- Screening of anti-IL-1α or anti-IL-1β antibodies
Download our Practical guide on Inflammasomes
Reference:
1. Dinarello C., 2018. Overview of the IL-1 family in innate inflammation and acquired immunity. Immunol Rev. 281(1): 8–27.
Back to the topSpecifications
Antibiotic resistance: Blasticidin, Hygromycin B, and Zeocin®
Growth Medium: DMEM, 4.5 g/l glucose, 2 mM L-glutamine, 10% (v/v) heat-inactivated fetal bovine serum, 100 U/ml penicillin, 100 µg/ml streptomycin, 100 µg/ml Normocin™
Guaranteed mycoplasma-free
Detection range for human IL-1β: 0.1 pg - 100 pg/ml
Detection range for human IL-1α, murine IL-1α, and murine IL-1β: 1 pg - 100 pg/ml
This product is covered by a Limited Use License (See Terms and Conditions).
Back to the topContents
- 1 vial containing 3-5 x 106 cells
- 2 x 1 ml of HEK-Blue Selection (250X)
- 1 ml of Normocin™ (50 mg/ml)
- 1 ml of QB reagent and 1 ml of QB buffer (sufficient to prepare 100 ml of QUANTI-Blue™ Solution, a SEAP detection reagent)
Shipped on dry ice (Europe, USA, Canada and some areas in Asia)
Details
IL‑1 is a secreted pro‑inflammatory cytokine that plays a critical role in immune responses and inflammation [1]. It exists in two distinct isoforms, IL-1α and IL-1β, which are produced as pro-proteins by activated macrophages. IL-1β is cleaved by caspase-1 [2] while IL-1α is cleaved by calcium‑activated calpain or caspase-5 (or its murine ortholog caspase-11) [3]. In contrast to pro‑IL‑1β, pro-IL-1α is active, although cleavage dramatically enhances its bioactivity. IL-1β and IL-1α bind to the same receptor, IL-1R1, triggering the formation of the IL-1R1/IL-1R3/MyD88 complex. This induces signaling leading to the activation of the transcription factors NK-κB and AP-1 with the subsequent inflammatory response [4].
1. Dinarello C., 2018. Overview of the IL-1 family in innate inflammation and acquired immunity. Immunol Rev. 281(1): 8–27.
2. Lopez-Castejon G. & Brough D., 2011. Understanding the mechanism of IL-1β secretion. Cytokine Growth Factor Rev. 22(4):189-95.
3. Wiggins K.A. et al., 2019. IL-1α cleavage by inflammatory caspases of the noncanonical inflammasome controls the senescence-associated secretory phenotype. Aging Cell. 18(3):e12946.
4. Weber A. et al., 2019. Interleukin-1 (IL-1) pathway. Sci Signal. 3(105):cm1.