HEK-Blue™ IL-1R Cells
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Human & Murine IL-1β Sensor Cells
3-7 x 10e6 cells
Human & Murine IL-1 Sensor Cells
HEK-Blue™ IL-1R cells were designed to detect bioactive human and murine cytokines (IL-1α and IL-1β) by monitoring the activation of the NF-κB and AP-1 pathways. Additionally, these cells detect bioactive IL-1β from cynomolgus monkeys, dogs, and rats. HEK-Blue™ IL-1R cells can detect IL-1α and IL-1β because these cytokines bind to the same receptor, IL-1R1.
These cells derive from HEK-Blue™ IL-1β cells in which the TNF-α response is blocked. Therefore, HEK-Blue™ IL-1R cells respond specifically to IL-1.
HEK-Blue™ IL-1R cells endogenously express the human IL-1 receptor and were stably transfected with the murine IL-1 receptor rendering these cells very sensitive to both human and murine IL-1β.
HEK-Blue™ IL-1R cells express a SEAP reporter gene under the control of the IFN-β minimal promoter fused to five NF-κB and five AP-1 binding sites. Binding of IL-1β to its receptor IL-1R on the surface of HEK-Blue™ IL-1R cells triggers a signaling cascade leading to the activation NF-κB and the subsequent production of SEAP.
Detection of SEAP in the supernatant of HEK-Blue™ IL-1R cells can be readily assessed using QUANTI-Blue™, a SEAP detection medium. QUANTI-Blue™ turns blue in the presence of SEAP which can be easily quantified using a spectrophotometer.
HEK-Blue™ IL-1R cells were incubated with increasing concentrations of recombinant human IL-1α or IL-1β, or mouse IL-1α or IL-1β. After 24h incubation the levels of NFкB-AP1- induced SEAP were determined using QUANTI-Blue™.
These cells can be used to detect human and murine IL-1 cytokines (both IL-1α and IL-1β).
Note: HEK-Blue™ IL-1R do not respond to human TNF-α but do respond to murine TNF-α.
Growth Medium: DMEM, 4.5 g/l glucose, 2 mM L-Glutamine, 10% (v/v) heat-inactivated fetal bovine serum (30 min at 56ºC), 50 U/ml penicillin, 50 µg/ml streptomycin, 100 µg/ml Normocin™
Detection range for hIL-1β: 1 pg - 100 ng/ml
Detection range for mIL-1β: 1 pg - 100 ng/ml
- HEK-Blue™ IL-1R cells were stimulated with various cytokines (IFN-α, IFN-β, IFN-γ, IL-1β, IL-4, IL-6, IL-10, IL-12, IL-13 and TNF-α).
- Production of SEAP was detected only following stimulation by IL-1β.
- 1 vial containing 3-5 x 106 cells
- 100 μl Hygromycin B Gold (100 mg/ml)
- 100 μl Puromycin (10 mg/ml).
- 100 μl Zeocin™ (100 mg/ml)
- 1 ml Normocin™ (50 mg/ml)
- 1 pouch of QUANTI-Blue™
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