HEK-Blue™ IL-18 Cells
|HEK-Blue™ IL-18 cells||Unit size||Cat. code||Docs||Qty||Price|
Human & Mouse IL-18 Reporter Cells
3-7 x 10e6 cells
IL-18 Reporter Cells
HEK-Blue™ IL-18 cells are designed to detect bioactive IL-18 by monitoring the activation of the NF-κB and AP-1 pathways. They were generated by stable transfection of HEK293 derived cells with the genes encoding IL-18R and IL-18RAP.
In addition, the TNF-α and the IL-1β responses have been blocked. Therefore, HEK-Blue™ IL-18 cells respond specifically to IL-18. These cells express an NF-κB/AP-1-inducible SEAP reporter gene.
Binding of IL-18 to the heterodimeric IL-18 receptor on the surface of these cells triggers a signaling cascade leading to the activation NF-κB and the subsequent production of SEAP.
Levels of SEAP in the supernatant can be easily determined with QUANTI-Blue™.
Growth medium: DMEM, 4.5 g/l glucose, 2 mM L-glutamine, 10% (v/v) heat-inactivated fetal bovine serum (30 min at 56°C), 50 U/ml penicillin, 50 μg/ml streptomycin, 100 μg/ml Normocin™.
Specificity: Detects human & murine IL-18.
Detection range: 10 pg - 1 ng/ml for human IL-18, 3 ng - 100 ng/ml for mouse IL-18.Back to the top
- 1 vial containing 3-7 x 106 cells.
- 2 x 1 ml HEK-Blue™ Selection (250X concentrate); a solution containing several selection antibiotics.
- 1 ml Normocin™ (50 mg/ml). Normocin™ is a formulation of three antibiotics active against mycoplasmas, bacteria and fungi.
- 1 pouch of QUANTI-Blue™ (SEAP detection medium).
Shipped on dry ice (Europe, USA & Canada)Back to the top
Interleukin-18 (IL-18) is a pro-inflammatory cytokine that causes a wide variety of biological effects associated with infection, inflammation and autoimmune processes.
It binds to an heterodimeric receptor consisting of IL-18R and IL-18 receptor accessory protein (IL18RAP) which then induces signaling pathways that involve MyD88, and TRAF-6 leading to the activation of NF-κB and AP-1.Back to the top