TSLP Reporter HEK 293 Cells
Product | Unit size | Cat. code | Docs. | Qty. | Price | |
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HEK-Blue™ TSLP Cells Human TSLP Reporter Cells |
Show product |
3-7 x 10e6 cells |
hkb-tslp
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HEK-Blue™ TSLP vial Additional cell vial |
Show product |
3-7 x 10e6 cells |
hkb-tslp-av
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Notification: Reference #hkb-tslp-av can only be ordered together with reference #hkb-tslp.
Thymic Stromal Lymphopoietin Reporter Cells
Signaling pathway in HEK-Blue™ TSLP cells
HEK-Blue™ TSLP cells were engineered from the human embryonic kidney HEK 293 cell line to detect bioactive thymic stromal lymphopoietin (TSLP) by monitoring the activation of the JAK/STAT5 pathway. They can also be used for screening antibodies or small molecule inhibitors targeting the TSLP pathway.
TSLP is an interleukin-7 (IL-7)-like cytokine with multiple functions, including cell proliferation and survival. It is best known as a critical mediator of type 2 immune responses [1].
Cell line description
HEK-Blue™ TSLP cells were generated by stable transfection with the gene encoding for the TSLPR and IL-7Ra chains of the human TSLP receptor, STAT5, and a STAT5-inducible secreted embryonic alkaline phosphatase (SEAP) reporter. The binding of TSLP to its receptor triggers a signaling cascade leading to the activation of STAT5 and the subsequent production of SEAP. This can be readily assessed in the supernatant using QUANTI-Blue™ Solution, a SEAP detection reagent.
HEK-Blue™ TSLP cells detect human but not murine TSLP (see figures). Of note, these cells also respond, to a weaker extent, to human IFN-γ (see figures). However, they do not respond to other STAT5-signaling cytokines: IL-2, IL-7, IL-9, and IL-15 (see figures).
Key features
- Fully functional TSLP signaling pathway
- Readily assessable STAT5-inducible SEAP reporter activity
- Strong response to human (h) TSLP
- No response to murine (m) TSLP
- Weak response to hIFN-γ
- No response to hIL-2, hIL-7, hIL-9, and hIL-15
Applications
- Detection and quantification of human and murine TSLP activity
- Screening of anti-TSLP and anti-TSLP receptor antibodies
- Screening of small molecule inhibitors of the TSLP pathway
Reference:
1. Ebina-Shibuya R. and Leonard WJ., 2023. Role of thymic stromal lymphopoietin in allergy and beyond. Nat Rev Immunol, 23(1):24-37.
Back to the topSpecifications
Antibiotic resistance: Blasticidin, Hygromycin B, Zeocin®
Growth medium: DMEM, 4.5 g/l glucose, 2 mM L-glutamine, 10% (v/v) heat-inactivated fetal bovine serum, 100 U/ml penicillin, 100 μg/ml streptomycin, 100 μg/ml Normocin®
Specificity: Detects human TSLP
Detection range:
- Detection range for human TSLP: 0.3 - 100 ng/ml
Quality Control:
- SEAP reporter activity in response to TSLP is validated using functional assays.
- The stability for 20 passages following thawing is confirmed.
- These cells are tested for mycoplasma contamination.
Contents
- 1 vial containing 3-7 x 106 cells
- 2 x 1 ml HEK-Blue™ Selection (250X concentrate)
- 1 ml Normocin® (50 mg/ml)
- 1 ml of QB reagent and 1 ml of QB buffer (sufficient to prepare 100 ml of QUANTI-Blue™ Solution, a SEAP detection reagent)
Shipped on dry ice (Europe, USA, Canada, and some areas in Asia)
Back to the topDetails
Thymic stromal lymphopoietin (TSLP) is a cytokine produced by epithelial and stromal cells in barrier tissues as well as innate immune cells such as dendritic cells, basophils and mast cells. It has pleiotropic actions on a wide range of immune and non-immune cell types [1]. TSLP belongs to the common γ chain (γc) cytokine family. Its receptor comprises the IL-7Rα (CD127) and TSLPR (CRLF2), a γc-like subunit. It signals through tyrosine kinases of the Janus family (JAK1 and JAK2) and signal transducer and transcription activators (STATs), notably STAT5 [1]. TSLP is a pleiotropic cytokine best known as a critical mediator of type 2 immune responses and a promoter of Th2 cell-mediated diseases (e.g. asthma) [1,2]. Emerging evidence indicates that TSLP is also implicated in viral infections, cancer, chronic inflammation and fat metabolism [1].
1. Ebina-Shibuya R. and Leonard WJ., 2023. Role of thymic stromal lymphopoietin in allergy and beyond. Nat Rev Immunol, 23(1):24-37.
2. Schmitt P. et al., 2024. TL1A is an epithelial alarmin that cooperates with IL-33 for initiation of allergic airway inflammation. J Exp Med. 221(6) e20231236.