Invivogen
Menu

IL-17C Reporter HEK 293 Cells

HEK-Blue™ IL-17C Cells Unit size Cat. code Docs Qty Price
Human & Mouse IL-17C Reporter Cells
3-7 x 10e6 cells
hkb-il17c
+-
$1,304.00

You may also need : IL-17 Reporter HEK 293 Cells | View more associated products

IL-17C Reporter Cells

HEK-Blue IL-17 Cells signaling pathway
HEK-Blue™ IL-17C Cells signaling pathway

HEK-Blue™ IL-17C cells were engineered from the human embryonic kidney HEK293 cell line to detect bioactive human and murine interleukin-17C (IL-17C) by monitoring the activation of the NF-κB and AP-1 pathways.  In addition, these cells can be used for screening anti-IL-17C or anti-IL-17 receptor antibodies. IL-17C is a pro‑inflammatory cytokine that promotes Th17 immunity to pathogens in the lungs, skin, and colon [1].

More details More details on Interleukin 17

 

Cell line description:

HEK-Blue™ IL-17C cells were generated by stable transfection of the human genes encoding the IL-17RA/IL-17RE heterodimeric receptor and Act1 adaptor molecule. HEK-Blue™ IL-17C cells also express an NF-κB- and AP-1-inducible secreted embryonic alkaline phosphatase (SEAP) reporter gene. For your convenience, InvivoGen provides QUANTI‑Blue™ Solution, an easy and rapid means to detect and quantify SEAP activity in the cell culture supernatant. Of note, HEK-Blue™ IL-17C cells also respond to human and murine IL-17E (also known as IL-25), as well as human IL-17A. They display little to no response to human IL-17F and do not respond to murine IL-17A nor IL-17F.

Features of HEK-Blue™ IL-17C cells:

  • High sensitivity to h/mIL-17C & h/mIL-17E
  • Moderate sensitivity to hIL-17A
  • Low to no sensitivity to mIL-17A & h/mIL-17F
  • Readily assessable SEAP reporter activity
  • Functionally tested and guaranteed mycoplasma-free

Applications of HEK-Blue™ IL-17C cells:

  • Detection of h/mIL-17C
  • Detection of h/mIL-17E (IL-25)
  • Screening of anti-IL-17 or anti-IL-17 receptor antibodies

 

References:

1. Yamaguchi S. et al., 2018. The roles of IL-17C in T cell-dependent and -independent inflammatory diseases. Sci Rep. 8(1):15750.

Figures

HEK-Blue™ IL-17C Cells sensitivity to IL-17 cytokines
HEK-Blue™ IL-17C Cells sensitivity to IL-17 cytokines
Evaluation of cellular response to IL-17 cytokines in HEK-Blue™ IL-17C Cells
Evaluation of cellular response to IL-17 cytokines in HEK-Blue™ IL-17C Cells

Dose-response of HEK-Blue™ IL-17C cells to recombinant IL-17 cytokines.
Cells were stimulated with increasing concentrations of recombinant human or murine IL-17C, IL-17E, and IL-17A. After overnight incubation, the NF-κB response was determined using QUANTI‑Blue™, a SEAP detection reagent, and reading the optical density (OD) at 630 nm. EC50 values are shown as mean ± SD.

Evaluation of specificity in HEK-Blue™ IL-17C Cells
Evaluation of specificity in HEK-Blue™ IL-17C Cells

Cytokine response profile of HEK-Blue™ IL-17C cells. 
Cells were stimulated with various human and murine recombinant cytokines: 10 ng/ml of hIL‑17C, mIL‑17C, hIL‑17E, mIL‑17E, hIL‑17A, mIL-17A, 1x104 IU/ml of hIFN-α, hIFN-β, 100 ng/ml of hIFN-γ, and 1 ng/ml of hIL-1 β and hTNF-α. After overnight incubation, SEAP activity was assessed using QUANTI‑Blue™. 

Evaluation of IL-17 inhibition in HEK-Blue™ IL-17C Cells
Evaluation of IL-17 inhibition in HEK-Blue™ IL-17C Cells

Dose-dependent inhibition of human IL-17C response using a neutralizing antibody against the human IL-17RA receptor in HEK‑Blue™ IL-17C cells. 
The antibody was incubated with HEK-Blue™ IL-17C cells for 30 minutes prior to the addition of 30 ng/ml of hIL-17C. After overnight incubation, SEAP activity in the cell culture supernatant was assessed using QUANTI‑Blue™. Data represent % inhibition of reporter activity.

Back to the top

Specifications

Antibiotic resistance: Blasticidin, Hygromycin, Zeocin®

Growth medium: DMEM, 4.5 g/l glucose, 2-4 mM L-glutamine, 10% (v/v) heat-inactivated fetal bovine serum, 100 U/ml penicillin, 100 μg/ml streptomycin, 100 μg/ml Normocin™

Guaranteed mycoplasma-free

Specificity: human and mouse IL-17C & IL-17E (IL-25), and human IL-17A 

Detection range:

  • 3 - 100 ng/ml for human and mouse IL-17C
  • 0.3 - 100 ng/ml for human and mouse IL-17E (IL-25)
  • 10 - 100 ng/ml for human IL-17A

 

These cells are covered by a Limited Use License (See Terms and Conditions).

Back to the top

Contents

Dry ice shipping Shipped on dry ice (Europe, USA, Canada and some areas in Asia)

Back to the top

Details

Interleukin 17 (IL-17) is a family of six closely related cytokines (IL-17A to IL-17F) which can have both pro-inflammatory and anti-inflammatory activities. IL-17C is a pro‑inflammatory cytokine that promotes Th17 immunity to pathogens in the lungs, skin, and colon [1].

IL-17 cytokines exert their biological activities following binding to heterodimeric receptors containing the ubiquitous IL-17RA chain and a second IL-17R (C, B or E) chain. IL-17A and IL-17F bind to the IL-17RA/IL-17RC receptor, IL-17C binds to the IL-17RA/IL-17RE receptor, and IL-17E binds to the IL-17RA/IL-17RB receptor [1, 2]. The activated heterodimeric receptor recruits the Act1 adaptor and induces the TNF receptor-associated factor 6 (TRAF6) ubiquitylation. This triggers a signaling cascade that results in NF-κB and AP-1 activation [3].

 

1. Yamaguchi S. et al., 2018. The roles of IL-17C in T cell-dependent and -independent inflammatory diseases. Sci Rep. 8(1):15750.
2. Gu C. et al., 2013. IL-17 family: cytokines, receptors and signaling. Cytokine. 64(2):477-85.
3. Pappu R. et al., 2011. The interleukin-17 cytokine family: critical players in host defence and inflammatory diseases. Immunology. 134(1): 8–16.

Back to the top

FAQ Cell Lines

Visit our FAQ Any questions about our cell lines ? Visit our frequently asked questions page

Back to the top

Citations

Customer Service
& Technical Support
Shopping cart is empty